Monsanto Company

Centocor, Inc.

Harvard College

William R Tolbert

Joseph Feder

Mau-Jung Kuo

Richard C Kimes

Mary M Hitt

Raymond A Dwek

Thomas William Rademacher

Charles Lewis, Jr

Raj B Parekh

John Shanahan

Mark F Baumgartner

Moses J Folkman

Jitka V Olander

Raymond C Geimer

A tubular membrane suitable for the delivery of a gaseous fluid to a surrounding medium has an irregular exterior surface to facilitate the diffusion of gas from the lumen to the exterior.

Mass transfer membrane for oxygenation of animal cell reactors

Disclosed is a method of using a tubular membrane to deliver a gaseous fluid to a surrounding cell culture medium. The tubular membrane has an irregular exterior surface to facilitate the diffusion of gas from the lumen to the exterior.

Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co).

Tissue plasminogen activator oligosaccharide from normal human colon

Tissue plasminogen activator from normal human colon cells

Cell culture filter apparatus and method is disclosed for the large scale cultivation of mammalian cells. This system is adapted for convenient manual assembly - disassembly of internal parts and interchangeability of filtration units.

Cell culture filter apparatus and method

A method and apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product is disclosed. The cells are retained within a reactor vessel chamber in a semi-rigid matrix having interstices for passage of fluid nutrient medium. Fresh nutrient medium is supplied by perfusion into the matrix through relatively low porosity tubes which are suspended in the reactor chamber and which substantially traverse the matrix; expended medium and cell product is withdrawn through relatively high porosity tubes which also are suspended in the reactor chamber and which substantially traverse the matrix; and oxygenated gaseous medium is supplied by perfusion into the matrix through a selectively-permeable membrane disposed in the reactor chamber.

Static cell culture maintenance system

Monoclonal antibodies are isolated from spent culture medium of the in vitro growth of hybridoma cells by treating said spent cell culture medium or a concentrate thereof with a water-insoluble, cross-linked polyelectrolyte copolymer.

Method of isolating monoclonal antibodies from hybridoma cultures

A low trauma, reversible flow pumping system is disclosed which is useful for transfer of biological fluids containing fragile components such as cells. The pumping system comprises a length of collapsible and flexible tubing in fluid communication at each end with a two-way, gravity actuated check valve means having a self-centering, vertically slidable weight member for directional regulation of fluid flow. Said tubing is sealingly enclosed within a hydraulic fluid containing chamber which is in fluid communication with oscillatory pressure providing means to cause alternate expansion and contraction of said tubing.

Cell culture pumping system

Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor.

Method of growing anchorage-dependent cells

A cell culture system and apparatus is provided for the agitated suspension culturing of mammalian cells in a flask assembly in which the culture medium and cells are gently agitated by the slow rotation of agitator means suspended downwardly in the culture medium and having at least one flexible sheet with a relatively large surface area which is positioned on the agitator in a manner to allow it to billow like a sail as liquid spills from its trailing edge during said rotation of the agitator means.

Cell culture method and apparatus

Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.

Production of angiogenic factor by cell culture

Process for the production of human angiogenic factor in vitro comprising growing the human diploid cell line IMR-90 on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.

A quick connect coupler for fluid transfer is disclosed in which one or both of the mating coupler members is provided with a sleeve which encloses at least a portion of said coupler member adjacent to its coupling end. The sleeve has an inlet for admission of air through its outer wall, a narrow concentric gap between its inner wall and the enclosed coupler member at said coupling end, and a passageway leading from said air inlet to said concentric gap for passage of air over said coupling end. The coupler is adapted for repeated connecting and disconnecting by cooperating releasable coupling means without loss of sterility by virtue of an air shield which is produced over said coupling end by passage of sterile air through the concentric gap.

Quick connect coupler with air shield

Process for the production of human TAF in vitro comprising growing the human colon adenocarcinoma cell line HT-29 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Production of tumor angiogenesis factor by cell culture

Process for the production of human TAF in vitro comprising growing the human osteosarcoma cell line MG-63 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Growing community of inventors

Manchester, MO, United States of America

William R Tolbert