Metabolix, Inc.

Cj Cheiljedang Corporation

Cj Research Center LLC

William R Farmer

Thomas Martin Ramseier

Frank Anthony Skraly

Yossef Shabtai

Jeffrey A Bickmeier

Oliver P Peoples

Johan Van Walsem

Christopher L Mirley

Erik Anderson

Kevin A Sparks

John Licata

Himani Chinnapen

Melarkode S Sivasubramanian

Ann D'Ambruoso

Dong-Eun Chang

Chenfeng Lu

Jeff Bickmeier

Catherine Morse

Thomas M Ramseier

A polyhydroxyalkanoate copolymer composition is provided. The composition comprises a plurality of polyhydroxyalkanoate copolymer molecules. The polyhydroxyalkanoate copolymer molecules (i) comprise 3-hydroxybutyrate monomers and 4-hydroxybutyrate monomers, (ii) have a monomeric molar percentage of 4-hydroxybutyrate monomers of 23.5 to 75%, and (iii) have a biobased content of &#x2265;80%. Also provided is a method of making a polyhydroxyalkanoate copolymer composition. The method comprises culturing an organism in the presence of one or more carbon raw materials under conditions under which (a) the one or more carbon raw materials are converted to 3-hydroxybutyryl-CoA and 4-hydroxybutyryl-CoA and (b) the 3-hydroxybutyryl-CoA and the 4-hydroxybutyryl-CoA are polymerized to form the polyhydroxyalkanoate copolymer molecules, thereby forming the composition. The organism has been genetically engineered to comprise particular enzymatic activities, and to not comprise other particular enzymatic activities. The one or more carbon raw materials, taken together, have a biobased content of &#x2265;80%.

Polyhydroxyalkanoate copolymer compositions and methods of making the same

Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).

Green process and compositions for producing poly(5HV) and 5 carbon chemicals

Processes and methods for making biobased gamma-butyrolactone from renewable carbon resources are described herein.

Process for gamma-butyrolactone production

Growing community of inventors

Concord, MA, United States of America

William R Farmer