United States of America, As Represented by the Secretary of Agriculture

Demegen, Inc.

Other

The United States of America As Represented by the Secretary of

William R Belknap

Kent F McCue

David R Rockhold

Jesse M Jaynes

Joan Garbarino

Paul V Allen

Mary M Maccree

Sophie S Chang

Nathaniel T Taylor

Louise V T Shepherd

Howard V Davies

Mendel Friedman

Colleen M McMahan

James G Thomson

Roger L Thilmony

Eddie W Stover

Grisel P Ponciano

Teruko Osumi

Kenneth L Deahl

Joan Garbabino

Jose A Valdes Franco

Andrew Stapleton

Charles P Moehs

Yong Q Gu

Genetically altered guayule are generated which produce more rubber than the amount of rubber produced by a wild-type guayule. The genetically altered guayule plant contains an expression vector that encodes a protein involved in rubber production. Method of making and using the genetically altered guayule are included.

Genetically altered guayule having increased rubber production and methods thereof

Expression vectors and expression cassettes containing a tissue specific 5' transcription regulatory element, optionally linked to a translation regulatory element, optionally linked to an intron transcription regulatory element, operably linked to a heterologous polynucleotide encoding a protein or RNA of interest are described. The 5&#x2032; transcription regulatory elements control expression in root tissue cells, phloem tissue cells, or fruit and/or abscission zone tissue cells. These sequences are obtained fromplants. Methods of use of these expression vectors and expression cassettes are described, as well as genetically altered plants and parts thereof that contain these expression vectors and/or cassettes.

Citrus derived transcription and translation regulatory elements for tissue specific expression

The present invention relates to isolatedBul409 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul409 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-771 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul409 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul409 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

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The present invention relates to isolatedBul427 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul427 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-1154 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul427 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul427 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

Nucleic acid sequences from potato that encode the enzyme &#x3b2;-solanine/&#x3b2;-chaconine rhamnosyltransferase (SGT3) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT3 and thereby reduce levels of the predominant steroidal glycoalkaloids &#x3b1;-chaconine and &#x3b1;-solanine in Solanaceous plants such as potato are described.

Nucleic acid sequences from potato that encode the enzyme UDP-glucose:solanidine glucosyltransferase (SGT2) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT2 and thereby reduce the level of the more human-toxic of the two predominant steroidal glycoalkaloids &#x3b1;-chaconine and/or increase the level of the more insect-toxic &#x3b1;-solanine in Solanaceous plants such as potato are described.

Agene has been cloned from a late blight-resistance locus and its sequence provided, together with the sequence of the encoded protein. DNA encoding the resistance protein has been introduced into potato plants and confers resistance to, the causal agent of late blight. The resistance protein is in the class of Nucleotide Binding Site-Leucine-Rich Repeat Proteins (NBS-LRRP), and the gene inis flanked by related NBS-LRRP gene sequences.

Stabilized ubiquitin-lytic peptide fusion polypeptides and a method of making the same by sub-cloning nucleic acid sequences coding for lytic peptides into a plasmid vector comprising a promoter and ubiquitin polypeptide coding sequence, wherein the ubiquitin polypeptide sequence is linked to the 5&prime; end of the lytic peptide nucleic acid sequence and is translated as a fusion polypeptide.

Ubiquitin-lytic peptide gene promoter

Stabilized ubiquitin-lytic peptide fusion polypeptides and a method of making the same by sub-cloning nucleic acid sequences coding for lytic peptides into a plasmid vector comprising a promoter and ubiquitin polypeptide coding sequence, wherein the ubiquitin polypeptide sequence is linked to the 5' end of the lytic peptide nucleic acid sequence and is translated as a fusion polypeptide.

Plants producing lytic peptides

Ubiquitin-lytic peptide fusion gene constructs, protein products

DNA sequences from potato which encode the enzyme solanidine UDP-glucose glucosyltransferase (SGT) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of an antisense DNA construct to inhibit the production of SGT and thereby reduce glycoalkaloid levels in solanaceous plants, e.g., potato, are described.

Growing community of inventors

Albany, CA, United States of America

William R Belknap