Syngenta Participations Ag

Syngenta Crop Protection Ag

Syngenta (at) Limited

William Paul Bullock

Brent Delzer

Michael L Nuccio

Robert Arthur Dietrich

Todd Lee Warner

Timothy Kelliher

Suresh Babu Kadaru

Satya P Chintamanani

John Gass

Ulrich Stephan Hannappel

Satya Chintamanani

Katie Marie Hunter

Josh Severson

Kathy Jo Cook

Julie Lynne Ritland

Christopher Cody Hejlik

Provided here are methods of using a mutated patatin-like phospholipase IIα ('pPLAIIα,' renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Haploid induction compositions and methods for use therefor

Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Provided here are methods of using a mutated patatin-like phospholipase II&#x3b1; ('pPLAII&#x3b1;,' renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII&#x3b1; to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII&#x3b1;. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid&#x2014;inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

The present invention relates to a viability-retaining method of selecting corn embryos having a desired trait, said method comprising extracting embryos, removing associated non-embryogenic tissue or DNA from the exterior of the embryo; and extracting DNA directly from the embryo. The invention surprisingly shows that germinating embryos sampled directly for their DNA content are capable of continuing the germination process to form normal seedlings.

Method of selecting corn embryos

Provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit haploid induction (HI); methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

The invention relates to rapid and efficient methods and apparatuses for displacing target plant materials from seeds. In one embodiment, the invention relates to methods and apparatuses for displacing embryos from maize seeds. In yet another embodiment, the displaced embryos can be propagated and regenerated into plants.

Method and appartus for extraction of plant embryos

This invention relates to a method of transforming plant cells using whiskers on various types of target tissue. And further relates to a method of transforming plant cells using whiskers with an improved agitation method.

Growing community of inventors

Slater, IA, United States of America

William Paul Bullock