Conceivable Life Sciences Inc.

Tmrw Life Sciences, Inc.

William Nicholas Garbarini, Jr

Jacques Cohen

Gerardo Mendizabal-Ruiz

Alejandro Chavez-Badiola

Vladimir C Ocegueda Hernandez

Roberto Valencia-Murillo

Nuno Costa-Borges

William Alan Blair

Alan Murray

Adolfo Flores-Saiffe Farias

Cesar Millan

Timothy Alan Sharp

Amit Gupta

Kathryn Josephine Go

Johann Aguayo

Victor Manuel Medina Perez

Giuseppe Silvestri

Victor Manuel Rico Botero

Joshua Abram

Estefania Hernandez

José Gregorio Espinoza Figueroa

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence/machine learning system (AI/ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI/ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

Centrifuge-free sperm preparation in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform

A method for automated ICSI includes receiving at least one droplet containing an egg in a dish placed on a stage. The method includes using an artificial intelligence/machine learning system (AI/ML system) and an imaging system to detect a zona pellucida. The imaging system includes a microscopy system, a camera system, and a lighting system. The method includes holding the egg using a robotic microtool and lowering a robotic pipettor into the droplet. The method includes using the AI/ML system and imaging system to determine an area at which to hold the egg and positioning the robotic microtool to that area. The method includes using the AI/ML system and imaging system to instruct the robotic microtool to apply negative pressure to hold the egg to the robotic pipettor. The method includes using the AI/ML system and imaging system to determine a target location where zona ablation should be performed.

Autonomous insemination in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform

A system and method facilitates transfers of specimen containers (e.g., vials with caps) between storage cassettes and carrier cassettes. The storage cassettes are designed to be stored in cryogenic refrigerators while the carrier cassettes are designed to be temporarily stored in a portable carrier. Identification information is read from wireless transponders carried by the specimen containers. Visual mappings of the positions of the specimen container in the cassettes is provided. Presence and position of the specimen containers in the cassettes is verified, and alerts of inconsistencies provided along with corrective commands. Inventories of specimen container and even specific specimen holders are provided.

Apparatus to facilitate transfer of biological specimens stored at cryogenic conditions

A method of artificial-intelligence-based robotic vitrification includes placing at least one oocyte in a buffer or CPA solution. The method includes processing an image object produced by an imaging system, using an AI/ML system, to determine a location of a retrievable target oocyte. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target oocyte. The method includes confirming the robotic pipettor's location at the target physical orientation, using the AI/ML system. The method includes instructing the robotic pipettor to initiate contact with the retrievable target oocyte and apply negative pressure to secure the oocyte to the robotic pipettor. The method includes using a robotic microtool holder to lower a vitrification assembly or cryo-device of any other make into a buffer or CPA solution dish to a position in proximity to the target physical orientation.

Autonomous vitrification in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform

Growing community of inventors

Cranford, NJ, United States of America

William Nicholas Garbarini, Jr