Dana-farber-cancer Institute Inc.

Harvard College

Children's Medical Center Corporation

William M Shih

Wesley Philip Wong

Peng Yin

Dionis Minev

Ralf Jungmann

Darren Yang

Shawn M Douglas

Bhavik Nathwani

Christopher Wintersinger

Chenxiang Lin

Chao Li

James J Chou

Yonggang Ke

Steven Perrault

Elisha Krieg

Zhao Zhao

Frances M Anastassacos

Richard Guerra

Luvena L Ong

Thorsten Lars Schmidt

Mark Theilmann

Provided herein, in some embodiments, are methods, compositions and kits for controlling nucleation and assembly of molecular nanostructures, microstructures and macrostructures.

Crisscross cooperative self-assembly

The present disclosure provides, in some aspects, nucleic acid nanostructures covalently linked to oligolysine-PEG copolymers.

Nucleic acid nanostructures crosslinked with oligolysine

Provided herein, in some embodiments, are methods and compositions for the production of long single-stranded DNA.

DNA-tagged methanol responsive polymer for single-stranded nucleic acid production

Provided herein, in some embodiments, are methods, compositions and kits for large-scale production of long single-stranded DNA in solution.

Acrylamide copolymerization for sequestration and production of single-stranded nucleic acid

The present disclosure provides nucleic acid-based nanoswitch catenanes and methods of use. A nanoswitch catenane may include a single-stranded nucleic acid comprising a first and second terminal domain linked to each other to form a host ring by one of a first, second or third switchable bridges, wherein the first switchable bridge is formed in the presence of a reaction agent through the reaction of two cognate functional groups, each linked to a terminal domain of the single-stranded nucleic acid, wherein the second switchable bridge is formed in the presence of a biomolecule of interest through binding of the bio-molecule of interest to two cognate antibodies, each linked to a terminal domain of the single stranded nucleic acid, and wherein the third switchable bridge is a link between two cognate functional groups that breaks in the presence of a dissociation agent. A nanoswitch catenane may also include a circular nucleic acid guest ring catenated with the host ring.

Nucleic acid nanoswitch catenanes

Provided herein are compositions and methods for determining the structure of individual targets using by determining long-range distances within such targets.

High-throughput structure determination using nucleic acid calipers

Provided herein are, inter alia, barcode probes comprised of transiently or stably fluorescently labeled nucleic acid nanostructures that are fully addressable and able to be read using standard fluorescent microscope and methods of use thereof including methods of use as detectable labels for probes.

Nucleic acid nanostructure barcode probes

The invention involves the synthesis of nucleic acid structures of controlled size and shape and comprised of a plurality of oligonucleotides. The structures are formed, at least in part, by the self-assembly of single-stranded oligonucleotides. The location of each oligonucleotide in the resultant structure is known. Accordingly, the structures may be modified with specificity.

Self-assembly of nucleic acid nanostructures

Holder

Provided herein are compositions and methods for determining the structure of individual targets using nucleic acid caliper by determining long-range distances within such targets.

Provided herein are nucleic acids and methods for selectively amplifying in parallel tens of thousands of high quality oligonucleotides without common sequences. The resultant oligonucleotides can be used for a variety of purposes and applications including but not limited to DNA nano structure synthesis.

Selective nucleic acid amplification from nucleic acid pools

The invention provides nanoparticles containing a nucleic acid nanostructure, of defined shape and size, linked to a hydrophobic moiety and coated by lipids, compositions comprising the nanoparticles, and methods of producing and methods of using the nanoparticles.

Growing community of inventors

Cambridge, MA, United States of America

William M Shih