National Research Council of Canada

University of Saskatchewan

Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-Food

Her Majesty the Queen in Right of Canada as Represented by the Minister of Natural Resources

Id Biomedical Corporation

The Natural Research Council of Canada

William L Crosby

Hong Wang

Larry C Fowke

Gopalan Selvaraj

Raju S Datla

Faouzi Bekkaoui

Peter D Duck

Richard H Tullis

Joe K Hammerlindl

Methods for using cyclin-dependent kinase (CDK) inhibitor genes, or anti-sense constructs complementary to such genes, to modify the growth and development of plant cells and organs are disclosed. Also provided are methods of modifying the development of plant cells and plants by transforming plant cells with nucleic acids encoding cyclin-dependent kinase inhibitor polypeptides, or anti-sense constructs complementary to such nucleic acids, to produce transformed plant cells, and then culturing the plant cells or regenerating a plant under conditions wherein the cyclin-dependent kinase inhibitor, or the anti-sense construct, is expressed. A variety of CDK inhibitor genes, and corresponding anti-sense constructs, are disclosed for use in a variety of plants. The nucleic acid encoding the cyclin-dependent kinase inhibitor may be operably linked to a tissue-specific promoter. Other provided aspects are modified transgenic plants and plant tissues. Also provided are methods of identifying nucleic acids that encode cyclin-dependent kinase inhibitors that are active in plants to modify the development of the plant.

Cyclin-dependent kinase inhibitors as plant growth regulators

Briefly stated, the present invention provides novel compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules. Within one aspect of the present invention, oligonucleotide-enzyme fusion molecules are provided, comprising an enzyme capable of cleaving scissile linkages and an oligonucleotide having the structure (NA.sub.1).sub.x --S.sub.z --(NA.sub.2).sub.y wherein NA.sub.1 and NA.sub.2 are nucleic acid sequences, S is a scissile nucleic acid linkage, x, y and z are integers from 1 to 100 and n is an integer from 1 to 10.

Compositions and methods for detecting target nucleic acid sequences

Bifunctional genetic markers

Growing community of inventors

Saskatoon, Canada

William L Crosby