Greenlight Biosciences, Inc.

Other

Gen9, Inc.

Westend Asset Clearinghouse Company, LLC

William Jeremy Blake

Drew S Cunningham

Daniel MacEachran

Matthew Eduardo Moura

James Robert Swartz

James Robbins Abshire

Mehak Gupta

Daniel Klein-Marcuschamer

Robert L Swanson

Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.

Cell-free production of sugars

Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Cell-free production of ribonucleic acid

Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence, wherein the recombinant proteins can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.

Engineered proteins with a protease cleavage site

Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

Iterative nucleic acid assembly using activation of vector-encoded traits

Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.

Engineered phosphoglucose isomerase proteins with a protease cleavage site

The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.

Cell-free system for converting methane into fuel and chemical compounds

Provided herein are cell-free systems for generating carbapenems, e.g., a compound of the Formula (I): or salts thereof; wherein, R, R, R, R, R, and Rare defined herein. Also provided are pharmaceutical compositions comprising a compound generated by the inventive cell-free system, and use of these compounds and compositions for the treatment of bacterial infections.

Cell-free preparation of carbapenems

An inner member is telescopically mounted within an outer tubular member and is normally locked against telescopic sliding movement relative to the outer member by a coupling mounted for back and forth manual manipulation along the outside of the two telescoping members at the location where the two members are coupled together. Manual manipulation of the coupling in one direction unlocks the inner member to permit sliding movement thereof relative to the outer member and the coupling, so long as the coupling is urged manually in the one direction. When the coupling is released, it automatically locks the inner member against further sliding movement.

Coupling for telescoping members

A telescoping, pole-type lure retriever has a snare portion that wraps around a fishing line and is guided thereon for removing a fishing lure that is snagged on an underwater obstruction or weeds. An elongated pole has a number of telescoping sections that may be extended to a convenient length to disengage the lure. Each telescoping section may be locked in position by turning a locking nut that compresses a locking ring in a cammed fashion to grip a smaller diameter pole section. The snake portion has a knurled shank seated in locking engagement with a sleeve member and with the sleeve fitted in the end of one pole section.

Telescoping device for retrieving fishing lures

A waste gate for an internal combustion engine having an exhaust gas driven supercharger supplying air under pressure to the engine intake manifold which is sensitive to the intake manifold pressure and reduces the quantity of air supplied to the intake manifold by the supercharger when the pressure in the intake manifold attains a predetermined value for efficient use of fuel supplied to the engine and increasing its power output.

Growing community of inventors

Winchester, MA, United States of America

William Jeremy Blake