Intrexon Corporation

Precigen, Inc.

Oakbio, Inc.

William J Coleman

Eli S Groban

Sheela Muley

Genevieve M Vidanes

Guillaume Cottarel

Roy Kamimura

Akbar F Javan

Jianping Sun

Brian Sefton

Robert David Castagno

Chia-Han Song

A crtW gene from a strain ofis disclosed that encodes a novel ketolase for carotenoid synthesis. An exemplary synthetic operon containing additional relevant carotenoid gene sequences is also provided, where the expression of the synthetic operon is used to produce ketocarotenoids. Suitable DNA expression constructs derived from these sequences are inserted into an expression host for expression. The expression product is a ketolase enzyme that is operable for transforming beta-carotene into canthaxanthin and astaxanthin.

Methods for producing biochemicals using enzyme genes derived from a strain of, and compositions made thereby

Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source 'farmland' currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions.

Biological production of multi-carbon compounds from methane

Aquafeed, animal feed, and other food products, as well as nutritional and pharmaceutical compounds, chemicals and biomaterials are important commodities that can be produced at commercial scale by fermentation of microorganisms. The present invention provides a method for producing these valuable multi-carbon compounds from simple gas feedstocks, such as carbon dioxide, hydrogen and oxygen, by cultivating a consortium of microbial cells specially selected for this purpose in an aqueous culture medium. In addition to exploiting inexpensive feedstocks, such as waste industrial gas for this cultivation, the platform described herein also provides the advantage of removing carbon dioxide and other waste gases from industrial emissions, which would otherwise contribute to global climate change. Furthermore, the cultivation of a microbial consortium can provide highly nutritious components to a feed blend that might not be available from a monoculture.

Microbial biomass based feed products

Hair treatment applicator

Growing community of inventors

Redwood City, CA, United States of America

William J Coleman