The Brigham and Women's Hospital, Inc.

The Charles Stark Draper Laboratory, Inc.

William J Adams, Jr

Jeffrey T Borenstein

Jason Oliver Fiering

Eli J Weinberg

Ernest Soonho Kim

Peter Mack

Guillermo Garcia-Cardena

Ahmad S Khalil

Stephen L Bellio

Mitchell L Hansberry

The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.

Systems and method for high-throughput testing

A high-throughput flow system includes an array of wells and a separate mechanical tip positioned within each well. Each mechanical tip is separately actuated to impart a shear stress pattern. A separate sleeve may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable within its corresponding sleeve. Alternatively, a separate post may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable about its corresponding post.

High-throughput biological screening

Growing community of inventors

Cambridge, MA, United States of America

William J Adams, Jr