Novo Industri A/s

Novo Laboratories, Incorporated

William H McMullen

William Carasik

Rosemary Andino

Expanded bed isomerization of glucose syrup to a glucose-fructose mixture having a fructose proportion exceeding about 40% of the glucose-fructose content by passing 30-55% w/w of glucose syrup through a cell-mass glucose isomerase preparation in the form of a bed of uniform particles of controlled size in the range of 150-2000 micrometers at a superficial syrup velocity of 0.5-15 meters an hour. Velocity and particle size are inversely related to create bed expansion within the range of 5-100%. Bed life may be extended by substituting fresh catalyst for inactivated catalyst. The isomerase should have an activity exceeding about 100 IGIC u/gm. The isomerase should be weighted by incorporation therein of finely divided alumina particles.

Glucose isomerization under expanded bed conditions

Saccharification of 10-20% solid content dextrin solutions for 15-75 hours at amyloglucosidase enzyme dosage levels of 0.3-1.0 AG u/g of solids results in glucose syrups with purity levels exceeding 98%. Glucose purity levels in excess of 98.5% can be achieved if the dextrin solution is formed by enzymatic liquefaction of starch, and saccharification is carried out for 15-25 hours with 0.4-0.8 AG u/g.

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Norwalk, CT, United States of America

William H McMullen