Genentech, Inc.

City of Hope

William E Holmes

Richard L Vandlen

William I Wood

James M Lee

Herbert L Heyneker

Arthur D Riggs

Ronald B Wetzel

Shmuel Cabilly

Deborah L Higgins

Adair J Hotchkiss

Gordon A Vehar

The invention relates to processes for producing an immunoglobulin or an immunologically functional immunoglobulin fragment containing at least the variable domains of the immunoglobulin heavy and light chains. The processes can use one or more vectors which produce both the heavy and light chains or fragments thereof in a single cell. The invention also relates to the vectors used to produce the immunoglobulin or fragment, and to cells transformed with the vectors.

Methods of making antibody heavy and light chains having specificity for a desired antigen

Novel 2 polypeptides with binding affinity for the p185receptor, designated heregulin 2-&#x3b1; and heregulin 2-&#x3b2;, have been identified and purified from human tissue. The cDNA encoding the novel heregulin 2-&#x3b1; has been isolated from human tissue and sequenced. Provided herein is nucleic acid sequence of the heregulin 2-&#x3b1; useful in the production of heregulin 2-&#x3b1; by recombinant means. Further provided an amino acid sequence of heregulin 2-&#x3b1; and heregulin 2-&#x3b2;. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

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Antibodies to heregulin 2

Novel polypeptides with binding affinity for the p185 receptor, designated heregulin 2-&agr; and heregulin 2-&bgr;, have been identified and purified from human tissue. The cDNA encoding the novel heregulin 2-&agr; has been isolated from human tissue and sequenced. Provided herein is nucleic acid sequence of the heregulin 2-&agr; useful in the production of heregulin 2-&agr; by recombinant means. Further provided an amino acid sequence of heregulin 2-&agr; and heregulin 2-&bgr;. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

Structure, production and use of heregulin 2 ligands

Methods of producing immunoglobulins, vectors and transformed host cells for use therein

Novel polypeptides with binding affinity for the p185.sup.HER2 receptor, designated heregulin 2-.alpha. and heregulin 2-.beta., have been identified and purified from human tissue. The cDNA encoding the novel heregulin 2-.alpha. has been isolated from human tissue and sequenced. Provided herein is nucleic acid sequence of the heregulin 2-.alpha. useful in the production of heregulin 2-.alpha. by recombinant means. Further provided an amino acid sequence of heregulin 2-.alpha. and heregulin 2-.beta.. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

Antibodies specific for heregulin 2-.alpha.

Novel 2 polypeptides with binding affinity for the p185.sup.HER2 receptor, designated heregulin 2-.alpha. and heregulin 2-.beta., have been identified and purified from human tissue. The cDNA encoding the novel heregulin 2-.alpha. has been isolated from human tissue and sequenced. Provided herein is nucleic acid sequence of the heregulin 2-.alpha. useful in the production of heregulin 2-.alpha. by recombinant means. Further provided an amino acid sequence of heregulin 2-.alpha. and heregulin 2-.beta.. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

Method of using HRG2-.alpha. to stimulate P185.sup.HeR2

The cDNA encoding a member of or a class of novel PF4A receptors has been identified in human tissue. Provided herein is nucleic acid sequence of a PF4A receptor (the IL-8 receptor) useful as a diagnostic and in the recombinant preparation of PF4A receptors. The PF4A receptor is used in the preparation and purification of antibodies capable of binding to the receptor, and in diagnostic assays.

Nucleic acids encoding a human IL-8 receptor

A novel polypeptide with binding affinity for the p185.sup.HER2 receptor, designated heregulin-.alpha., has been identified and purified from cultured human cells. DNA sequences encoding additional heregulin polypeptides, designated heregulin-.alpha., heregulin-.beta.1, heregulin-.beta.2, heregulin-.beta.2-like, and heregulin-.beta.3, have been isolated, sequenced and expressed. Provided herein are nucleic acid sequences encoding the amino acid sequences of heregulins useful in the production of heregulins by recombinant means. Further provided are the amino acid sequences of heregulins and purification methods therefor. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

Growing community of inventors

Pacifica, CA, United States of America

William E Holmes