Emd Millipore Corporation

The Provost, Fellows, Foundation Scholars, and the Other Members of Board, of the College of the Holy and Undivided Trinity of Queen Elizabeth Near Dublin

Merck Millipore Ltd.

Merck Patent Gmbh

William Cataldo

Mikhail Kozlov

Matthew T Stone

John Paul Amara

David Yavorsky

Joaquin A Umana

Dennis Aquino

Alex Xenopoulos

Jeffrey Caron

Wilson Moya

Gabriel Tkacik

James Hamzik

Ajish Potty

Jad Jaber

Michael W Phillips

Christopher Gillespie

Ramesh Babu Padamati

Kevin Galipeau

Clifton Ngan

Mikahil Kozlov

Kamran Beyzavi

Inga Elkina

Murugan Rajendiran

Michael Mansfield

Daniel Callahan

Michael Phillips

Joaquin Umana

Lars Peeck

Provided herein are electrospun or electroblown non-woven fiber membranes, methods of making such membranes and lateral flow diagnostic devices comprising such membranes.

Non-woven fiber membranes

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Chromatography media and method

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.

Purification of biological molecules

Embodiments described herein relate to electrospun nanofiber ultrafiltration membrane compositions capable of operating in tangential filtration mode and methods of using the same.

Method of purifying a biological material of interest in a sample using nanofiber ultrafiltration membranes operated in tangential flow filtration mode

Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

Protein separations using an acrylamide containing filter

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Bradford, MA, United States of America

William Cataldo