Washington State University

Other

The United States of America As Represented by the United States

University of Florida Research Foundation, Incorporated

The United States of America As Represented by the Secretary of

William C Davis

Travis C McGuire

Terry F McElwain

Guy H Palmer

Lance E Perryman

Donald P Knowles, Jr

Anthony F Barbet

Bernard John Van Wie

Douglas P Jasmer

Will L Goff

Moris L Silber

David W Reduker

Stephen A Hines

Bradley A Pomeroy

John Dahl

David F Moffett

Alan R Koch

Himawan Sutisna

Kun Taek Park

Steven R Reiken

Rudy Sanfelice

Mary Jo Hamilton

Christopher Sanfelice

Particular aspects provide efficient allelic exchange methods to generate directed mutations within genes of slow-growing stains of mycobacteria (e.g.,subsp.(Map), Map 10 or GFP-expressing Map K-10) using a phage-delivery system, and demonstrate high efficiency allelic exchange. Additional exemplary aspects provide non-naturally occurring slow-growing strains of mycobacteria (e.g., Map,) having at least one gene deletion (e.g., pknG, relA, lsr2, panC, panD, proC, trpD, sapM (MAP3432), lysA_1, leuD, and leuC, and deletion mutants at the orthologous loci of two known virulence genes in pathogenic mycobacteria (relA and pknG) and one gene related to colony morphology and biofilm formation in fast growing mycobacteria (lsr2) were made. Further aspects provide novel vaccines comprising such deletion mutants, or portions thereof, and methods for making said vaccines. Yet further aspects provide methods for protecting a mammal from virulent Map,, or, comprising treating the mammal with the inventive vaccines.

Allelic exchange mutagenesis in Map

A movable boring assembly and a boring tool positioner for drilling a plurality of holes in a well casing and jetting a well production zone through the plurality of holes. All of the holes can be drilled in the casing at different angular positions, as well as different levels within the casing. The drilling tool can then be removed and a perforation jet can be reliably and accurately reoriented into the drilled holes to perforate a production zone in multiple directions and at multiple levels.

Apparatus and method for positioning extended lateral channel well stimulation equipment

The subject invention concerns the use of the conserved,major surface protein 5 gene and gene product and monoclonal antibody ANAF16C1 for the identification of animals persistently infected with Anaplasma species.

Serological identification of cattle, sheep or goats infected with anaplasma species

The subject invention concerns the use of monoclonal antibody ANAF16C1 and Anaplasma species major surface protein-5 in the competitive inhibition format for the serological identification of animals infected with Anaplasma species.

Serological identification of cattle, sheep or goats infected with

The present invention relates to a biosensor for detecting the presence of an analyte in solution. The biosensor is composed of:

Biosensor for detecting the presence of chosen analytes

A purified antigenic surface protein of Anaplasma marginale has been identified, and is capable of inducing immune responses in ruminants which neutralizes virulent Anaplasma marginale. The antigenic surface protein has a molecular weight of about 105,000 daltons, and can be purified by an immunoaffinity chromatography process. The antigen has further utility in diagnostic tests for anaplasmosis. It can be synthesized by polypeptide procedures or by genetic engineering. DNA and amino acid sequences have been developed for the antigen according to this invention. The antigen is useful as a vaccine component for protecting mammals against infection by Anaplasma marginale and may be useful for rickettsial organisms other than Anaplasma marginale.

Immunogenic anaplasma marginale surface antigens, compositions, and

Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoclonal antibodies produced thereby. The gene encoding a 58 kD surface protein has been identified and the DNA sequence determined and compared with sequences of other known merozoite proteins. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monoclonal antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.

Immunization against babesiosis using purified surface antigens of

Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoclonal antibodies produced thereby. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monoclonal antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.

Immunization against Babesiosis using purified merozoite surface

The subject invention concerns the identification of novel merozoite surface proteins of Babesia bovis. Also disclosed are monoclonal antibodies to these proteins as well as genes which encode for the proteins. The invention further concerns the use of the novel proteins, recombinant DNA clones, and monoclonal antibodies in the detection, treatment, and prophylaxis of babesiosis.

Cloning of the Babesia bovis 60 KD antigen

Growing community of inventors

Pullman, WA, United States of America

William C Davis