The University of British Columbia

Illusense, Inc.

William Bowden

Anupam Singhal

Carl Lars Genghis Hansen

Veronique Lecault

Adam Keith White

Oleh Petriv

Tim Leaver

Dan Da Costa

Leo Wu

Georgia Russell

Darek Sikorski

Kirk William Madison

Kyzyl Herzog

Michael Vanlnsberghe

Michael VanInsberghe

Carl Lars Hansen

A method and an apparatus for determining a signed Doppler frequency shift of an optical signal. The method comprises generating a reference signal having a reference optical spectrum and a transmission signal having a transmission optical spectrum; directing the transmission signal to at least one target; receiving a reflection signal from the at least one target, the reflection signal having a reflection optical spectrum; estimating the reflection optical spectrum; and based on the estimated reflection optical spectrum and the reference optical spectrum, determining a signed Doppler frequency shift of the reflection optical spectrum, wherein at least one of the transmission optical spectrum and the reference optical spectrum comprises a first component having a first frequency and a first amplitude, and a second component having a second frequency, different from the first frequency, and a second amplitude, different from the first amplitude, and wherein the at least one of the transmission optical spectrum and the reference optical spectrum is asymmetric about the midpoint of the reference optical spectrum.

Method and system for simultaneous detection of signed doppler shifts and range measurements

Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Microfluidic cell trap and assay apparatus for high-throughput analysis

Growing community of inventors

Vancouver, Canada

William Bowden