Becton Dickinson and Company Limited

William B Brasso

Susan M Kircher

Adrien P Malick

Liping Feng

Vanda White

John D Mantlo

Song Shi

Ben Turng

Julie L Rosales

Jon E Salomon

Tuan-Linh Ngoc Nguyen

Xiao Mo

Dyan Luper

Patrick Shawn Beaty

David J Turner

James Y Zhou

Donald Ray Callihan

Curtis M Gosnell

Jeffery H Bruton

Fatimah Alrashidi-Brooks

John P Douglass

Gretta Campbell

John M Hejna

Gertrude M Karr

Denise R Holliday

Patricia Hammond

Donald R Callihan

Shawn Beaty

Provided herein are compositions, methods, systems and/or kits for detection of bacteria expressing enzymes that confer resistance to antimicrobial agents. Certain embodiments of the compositions, methods, systems and/or kits of the present disclosure are related to detection of carbapenemase-producing gram negative bacteria. Certain embodiments of the compositions, methods, systems and/or kits of the present disclosure are related to detection of Ambler Class A, B and/or D carbapenemase-producing enteric and non-fermenting gram negative rod bacteria.

Compositions, methods, systems and/or kits for detecting antimicrobial resistance in bacteria

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Formulations and process for isolating viable microorganisms from positive blood cultures

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture ('PBC') samples for use in downstream analyses such as identification and antimicrobial susceptibility testing ('AST'). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of

Method for direct and rapid identification of microorganisms and antimicrobial susceptibility testing from positive blood cultures

The present invention relates to a method for correcting false susceptibility results in antimicrobial susceptibility tests for resistant microorganisms. This method comprises adding specific amounts of sugars, carbohydrates, related compounds or other ingredients to a test medium for such susceptibility tests.

Addition of lactose or other sugars in correcting false susceptibility

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Columbia, MD, United States of America

William B Brasso