The United States of America, As Represented by the Secretary, Department of Health and Human Services

William Allan Nix

M Steven Oberste

Larry J Anderson

Thomas G Ksiazek

Anthony Sanchez

Pierre E Rollin

Cara Carthel Burns

Raymond Campagnoli

Paul A Rota

Mark A Pallansch

Suxiang Tong

James A Comer

Dean D Erdman

Cynthia Goldsmith

William J Bellini

Stephan S Monroe

Shannon L Emery

Charles D Humphrey

Joseph P Icenogle

Teresa C T Peret

Sherif R Zaki

Qi Chen

Jeanette Guarner

Richard F Meyer

Christopher D Paddock

Michael D Bowen

Wun-Ju Shieh

Byron T Cook

Methods and compositions for detection ofD in a sample, particularly detection ofD68, are provided. The methods include contacting a sample with at least one primer (such as a forward primer and/or a reverse primer) capable of specifically amplifying an EV-D68 viral protein 1 (VP1) nucleic acid or a portion thereof and/or a detectably labeled probe capable of specifically hybridizing to an EV-D68 VP1 nucleic acid, under conditions sufficient for specific amplification of the EV-D68 VP1 nucleic acid by the at least one primer and/or under conditions sufficient for specific hybridization of the probe to the EV-D68 nucleic acid. The amplification of the EV-D68 VP1 nucleic acid and/or the hybridization of the probe to the EV-D68 VP1 nucleic acid is detected, thereby identifying presence of EV-D68 in the sample.

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The present disclosure provides methods that permit detection of all known species of Parechovirus, including Human parechovirus and Ljungan virus (LV). In particular examples the method includes amplifying at least a portion of the 5'NTR of parechovirus nucleic acid molecules obtained from a sample, and detecting the resulting amplicons, but does not require culturing of the virus. The present disclosure also provides methods for determining which particular species or serotype of parechovirus is present in a biological sample. Also provided are oligonucleotide primers and probes that can be used in the disclosed methods.

Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.

Coronavirus isolated from humans

Disclosed are methods of using enterovirus-specific primers for the detection and identification of enterovirus infection. Also provided are isolated nucleic acid molecules and kits useful for detection and diagnostic testing of enterovirus infection in a subject.

Nucleic acid molecules and kits including VP1 and VP3 nucleic acid molecules, useful for detecting and identifying enteroviruses

Detection and identification of enteroviruses by semi-nested amplification of the enterovirus VP1 protein

Growing community of inventors

Bethlehem, GA, United States of America

William Allan Nix