Gist-brocades N.v.

Mogen International

Plant Genetic Systems N.v.

Wilhemus J Quax

Andreas Hoekema

Jan Pen

Krijn Rietveld

Albert J Van Ooijen

Teunis Cornelis Verwoerd

Cornelis J Van Der Laken

Cornelis P Broekhuizen

Johanna Hgm Mutsaers

Jan Metske Van Der Laan

Ignace Lasters

Peter Christiaan Sijmons

Nadir Mrabet

Peter Christian Sijmons

Rudolf Gm Luiten

Anne-Marie Lambeir

Paul W Schuurhuizen

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Production of enzymes in seeds and their use

Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.

Stabilization of carboxyl esterase

New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.

Glucose isomerase enzymes and their use

A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered substrate specificity. New mutant glucose isomerases with an altered substrate specificity are provided according to this method. These altered properties are useful in starch degradation and in other sugar conversion reactions.

Glucose isomerases having altered substrate specificity

Growing community of inventors

Voorschoten, Netherlands

Wilhemus J Quax