Gist-brocades N.v.

Genencor International, Inc.

Plant Genetic Systems N.v.

Other

Dsm N.v.

Shell Internationale Research Maatschappij, B.v.

European Molecular Biology Laboratory

Gist-brocades B.v.

National University of Ireland

Genecor International, Inc.

Mogen International Nv

Syngenta Mogen B.v.

Plant Genetic Systems

University of Groningen

Gist-hrocades

Wilhelmus J Quax

Jan Metske Van Der Laan

Onno Misset

Robert Mark Caldwell

Ignace Lasters

Gijsbert Gerritse

Nadir Mrabet

Brian E Jones

Arthur F Marx

Andreas Hoekema

Peter Christiaan Sijmons

Jan Pen

Diane P Williams

Gareth T Phillips

Peter D Watts

Krijn Rietveld

Hein Simon Koger

Mauro A Bertola

Sara Iverson

Richard Kerkman

Cornelis J Van Der Laken

Brian W Robertson

Anne-Marie Lambeir

Cornelis P Broekhuizen

Patrick Stanssens

Herman B Lenting

Frits Goedegebuur

Roberta L Farrell

Marc Jozef Lauwereys

Pieter Van Solingen

Gerard Venema

Jan Maarten Van Dijl

Sierd Bron

Teunis Cornelis Verwoerd

Petrus Josephus Van Den Elzen

Margareta Adriana Herweijer

Adrianus Wilhelmus Vollebregt

Adriana Marina Riemens

Hugh W Morgan

Luis Serrano

Albert Johannes Joseph Van Ooyen

Albert Johannes Van Ooyen

Hilde E Smith

Peter Leonard Bergquist

Afshin Samali

Margaret M Mullally

Margaretha Adriana Herwijer

Robbert H Cool

Rudolf Franciscus Van Beckhoven

Wilhelmus Antonius Van Der Kleij

Eva E Szegezdi

Wilhelmina Theresia Herbes

Gregorio Fernandez-Ballester

Vidar Gronberg

Dean Moody

Roberta Lee Farrell

Simon Forster

McIver Daniel

Jan M Van Der Laan

Yves Laroche

Vicente R Tur

Shoshana Wodak

Gaston Matthyssens

Albert Martinus Van Der Sloot

Adriana M Riemens

The present invention relates to novel methods for the design of proteins, in particular, cytokines. These methods allow the stabilisation of such cytokines, as well as modification of their selectivity/specificity for their cognate receptors. The invention also relates to various modified proteins that have been designed by the methods of the invention.

Cytokine design

The present invention relates to nucleic acid sequences and amino acid sequences fordisulfide bond isomerases, Dsband Dsband methods for increasing the secretion of heterologous and homologous proteins in Gram-positive microorganisms.

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A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Production of enzymes in seeds and their use

Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from is removed by overexpressing the protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.

Secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it

The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid amino acid sequences for the secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.

Increasing production of proteins in gram-positive microorganisms

The present invention relates to nucleic acid sequences and amino acid sequence for disulfide bond isomerases, Dsb1 and Dsb2 and methods for increasing the secretion of heterologous and homologous proteins in gram-positive microorganisms.

Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same

The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in gram-positive microorganisms.

Increasing production of proteins in gram-positive microorganisms using SecG

Secretion factors for gram-positive microorganisms genes encoding them and methods of using it

A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a,polymerase, a,sigma 54 promoter, a,upstream activating sequence, the,secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

Expression system for altered expression levels

The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid amino acid sequences for the,secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.

A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a,, a,sigma 54 promoter, a,upstream activating sequence, the,secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

Alkalitolerant xylanases

Thermostable xylanases

A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

New mutant .beta.-lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.

Mutated penicillin G acylase genes

Growing community of inventors

Voorschoten, Netherlands

Wilhelmus J Quax