Behringwerke Aktiengesellschaft

Hoechst Aktiengesellschaft

Wilhelm Winckler

Hans Bohn

A protein PP.sub.18 is isolated having the following characteristics:

Isolated tissue protein PP.sub.18

Tissue protein PP.sub.19, a process for obtaining it, and its use

The protein PP.sub.21 which has the following characteristics:

Tissue protein PP.sub.21, a process for obtaining it and its use

The protein PP.sub.20 is described, this having the following characteristics:

Protein PP.sub.20, a process for obtaining it, and its use

The protein PP.sub.17, which has (a) an electrophoretic mobility in the range between .beta..sub.1 - and .alpha..sub.2 -globulins, (b) an isoelectric point of 5.25.+-.0.20, (c) a sedimentation coefficient S.sub.20.sup.o, w of 2.7.+-.0.1 S, (d) a molecular weight, determined in polyacrylamide gel containing sodium dodecyl sulfate (SDS), of 38,000.+-.2,000, (e) an extinction coefficient E.sub.1cm.sup.1% (280 nm) of 8.5.+-.0.4 and (f) a carbohydrate content of 2.1.+-.0.9% containing 0.3.+-.0.2% of mannose, 0.4.+-.0.2% of galactose, 0.2.+-.0.1% of xylose, 1.0.+-.0.3% of N-acetyl-glucosamine and 0.2.+-.0.1% of N-acetyl-neuramin acid, is described. A process for concentrating and isolating it and its use are also described.

Protein (PP.sub.17), a process for concentrating and isolating it and

A new glycoprotein is characterized by a protein consisting essentially of 75.+-.6% of .alpha.-amino-acids, a carbohydrate portion of 24.6.+-.5.2%, which contains 8.9.+-.2% of hexoses, 7.1.+-.1.5 of N-acetylated hexosamine, 0.2.+-.0.2% of fucose, 8.4.+-.1.5% of, N-acetylated neuraminic acid; a sedimentation coefficient S.sub.20w of 2.5.+-.0.3 S; a molecular weight of 35,000.+-.5,000, determined in the ultracentrifuge, and a molecular weight of 65,000.+-.10,000, determined in polyacrylamide gel containing sodium dodecyl sulfate; an iso-electric point at pH 3.4.+-.0.4; an extinction coefficient E.sub.1.sup.1%.sub.cm (280 nm) of 1.9.+-.0.3; an electrophoretic mobility in the range between that of .alpha..sub.1 and .alpha..sub.2 -globulins; and a specific immunologic reaction with an antibody specifically directed against the glycoprotein.

New glycoprotein and process for isolating it

The invention relates to a tissue specific protein, i.e. a substance which is a constituent of parenchymatous tissues, and to a process for preparing the same. The tissue protein is characterized by a protein proportion of 94.+-.3%, essentially consisting of .alpha.-amino acids,

Tissue specific protein and process for preparing same

What is disclosed is a glycoprotein having proteolytic, fibrinolytic, and thrombolytic properties and which can be used to prepare anti-sera useful as diagnostic agents, said glycoprotein being found in human blood serum and extracts of human placentas and being capable of isolation therefrom and which has the following properties:

Glycoprotein and process for isolating it

A derivative of the steroid-binding .beta.-globulin which can be obtained therefrom by treatment with neuraminidase.

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Wenkbach, Germany

Wilhelm Winckler