Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Wilhelm Kleider

Peter Sluka

Thomas Wild

Friederike Weber

Hans-Dieter Kraemer

Christoph Berding

Hans-Dieter KRÄMER

A method of analyzing a biological sample using an analyzer and an assay. The method comprises providing the assay for producing the signal. The assay has two or more predetermined number of components. Each of the predetermined components has a distinct relation between the intensive property and the signal. The method further comprises providing calibration samples with known values for the intensive property and measuring a calibration signal for each of the calibration samples. The method further comprises determining a calibration by fitting a calibration function to the calibration signal for each of the calibration samples and the known values for the intensive property. The calibration function is equivalent to a constant plus an exponential decay term for each of the predetermined number of components. The method further comprises measuring the signal of the sample using the analyzer and the assay, and calculating the intensive property using the calibration.

Calibration for multi-component assays

In order to analyze a medical sample while avoiding error contributions due to hemolysis, prior to the main reaction for a component present in the sample, a pre-reaction is produced and measured by which the degree of hemolysis in the sample is determined and the result of the sample to be determined that is subsequently obtained is corrected by this error contribution using the relationship (correlation) that has been found between the degree of hemolysis and the error contribution.

Methods for determining an analyte in a plasma or serum sample which may be contaminated with interfering substances resulting from hemolysis

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Uffing am Staffelsee, Germany

Wilhelm Kleider