Hoffmann-la Roche Inc.

Roche Diagnostic Systems, Inc.

Wilhelm Bannwarth

Jan Mous

Stuart Le Grice

Patrick Caspers

Arnold Trzeciak

Klaus Muller

Daniel Obrecht

Francis Muller

Alfred Grieder

Andreas Knierzinger

Fernand Gerber

Polypeptides which include at least one antigenic and/or immunogenic determinant of the envelope protein (env) of the HIV-2 virus and their production by recombinant DNA technology and the DNA sequences, expression vectors and transformed single-cell organisms used in this process are provided. A method for the detection of HIV antibodies or HIV viruses in human sera or in other biological fluids is also provided.

HIV-2 envelope polypeptides

There are described compounds of the formula ##STR1## Wherein the variables have been defined herein. The compounds are useful as research tools in the determination of biologically active peptides sequences and also potentially suitable as medicaments, some of them being useful in the prevention or control of the formation of blood platelet thrombi, and some compounds are useful as intermediates.

Tri- and tetracyclic compounds

Tri-and tetracyclic compounds

A process for the detection of a nucleic acid sequence in a homogeneous assay format using an energy transfer system is disclosed. This process utilizes a 5' labeled primer containing a selfcomplementary sequence in an amplification or extension process together with a subsequent detection step using a 3' labeled probe for the amplified or extended region. The labels will be close together in space after hybridizing the probe close to the short piece of double-stranded DNA resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified or extended product. A new primer for use in this process is also disclosed.

Detection of nucleic acids using a hairpin forming oligonucleotide

Energy-transfer systems which can be used, inter alia, for measuring distances within or between different molecules are described, comprising derivatives of lumazine and ruthenium, in particular derivatives of DNA or RNA sequences.

Ruthenium-lumazine energy transfer systems

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Rheinfelden-Beuggen, Germany

Wilhelm Bannwarth