University of Illinois

Biotechnology Research and Development Corporation

Wilfred A Van Der Donk

Huimin Zhao

William W Metcalf

Ryan Woodyer

Michael Simurdiak

Yuki Goto

Benjamin Thomas Circello

Ayse Okesli

Amanda L McClerren

Mark Walker

Kou-San Ju

Kenton J Hetrick

Jiangtao Gao

James R Doroghazi

Lisa E Cooper

Neha Garg

Weixin Tang

Tyler Johnannes

Xiao Yang

Junkal Zhang

Svetlana A Borisova

David P Labeda

Platforms, systems and methods are provided for identifying engineered lanthipeptide display peptides expressed in vivo from biological organisms.

Biosynthesis and engineering of lanthipeptides

The present invention relates to pharmaceutical compositions comprising a phosphonic acid compound, such as a natural product. Such compounds can include, such as an antibiotic or compound with other activity, derived from anstrain having a gene encoding pepM (phosphoenolpyruvate phosphomutase) or a pepM-dependent biosynthetic pathway. The present invention also relates to methods for treating or preventing or reducing the risk of a bacterial infection by administering a therapeutically effective or prophylactically effective amount of a phosphonic acid antibiotic, or a pharmaceutical composition containing such an antibiotic, to a patient or subject in need thereof. The present invention further relates to methods for isolating, purfying, and identifying such phosphonic acid compounds fromstrains.

Phosphonic acid compounds and screening method

The disclosure provides lantibiotics geobacillin I and geobacillin II from, compositions comprising the lantibiotics, and methods of use of the lantibiotics. Further disclosed are the sequences of lantibiotics geobacillin I and geobacillin I, and alternative structures of geobacillin I and geobacillin II comprising amino acid substitutions. Antimicrobial compositions comprising one or more isolated geobacillins and a pharmaceutically acceptable carrier, and methods of reducing reproduction of bacteria comprising administering to a subject a therapeutically effective amount of the antimicrobial composition are also provided.

Class I and II lantibiotics from geobacillus thermodenitrificans

The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.

Compositions and methods for the synthesis of APPA-containing peptides

The present invention relates to two-component lantibiotics isolated fromsp. Methods for producing said lantibiotics are provided, wherein dehydration and cyclization of the peptides is carried out by two substrate-specific modifying enzymes. Given the antimicrobial activity of the instant lantibiotics, methods for preventing or treating bacterial infections are also provided.

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A new xylose reductase encoding gene fromwas heterologously expressed inas a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.

Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.

Phosphite dehydrogenase mutants for nicotinamide cofactor regeneration

Growing community of inventors

Champaign, IL, United States of America

Wilfred A Van Der Donk