National Research Council of Canada

Other

Dow Agrosciences LLC

Wilfred A Keller

Gopalan Selvaraj

Fawzy Georges

Steven Fabijanski

Paul G Arnison

Joseph K Hammerlindl

Steven R Webb

Raju S S Datla

Jin-Zhuo Dong

Atta A K Hussain

Jitao Zou

David Charles Taylor

Raju S Datla

Atta Hussain

Ramesh B Nair

Samuel L MacKenzie

Vesna Katavic

Richard W Joy, Iv

Genetic constructs, transformation vectors and methods are taught for production of transgenic plants which can be selectively removed from a growing site by application of a chemical agent or physiological stress. The invention links a target gene for the trait of commercial interest to a conditionally lethal gene, which can be selectively expressed to cause plant death. By use of the genetic constructs, transformation vectors and methods of the present invention, invasion of environments and contamination of commercial non-engineered productions by transgenic plants can be avoided. Methods are also taught for transformation ofspecies.

Transgenic plants and methods for production thereof

In a method for altering a nutritional profile of a plant, a nucleic acid sequence is selected for its ability to encode a protein capable of modifying the utilization of a substrate in the sugar alcohol secondary metabolic pathway of a plant. The protein is non-native to the sugar alcohol secondary metabolic pathway. Plant cells are transformed with an expression cassette containing the nucleic acid sequence. Genetically altered plants are recovered from the plant cells, the plants having an altered nutritional profile relative to wild-type plants. Genetically modified plants having improved nutritional profiles, and obtainable by the above method, are also provided.

Methods and compositions for modifying levels of secondary metabolic compounds in plants

A plant, multicellular fragment of said plant or seed of said plant transformed with a nucleotide sequence of SEQ ID NO 1 or an allelic variant or a fragment thereof or a genetic equivalent thereof according to the degeneracy of the genetic code coding for a peptide having amyo-inositol 1-phosphate synthase activity, said plant, multicellular fragment or seed having reduced myo-inositol 1-phosphate synthase activity when compared with an equivalent untransformed plant, multicellular fragment or seed, such that there is reduced phytate present in the plant, multicellular fragment or seed. The invention also provides a method for reducing phytate in, which method comprises growing aplant comprising one of a myo-inositol 1-phosphate synthase antisense sequence and a myo-inositol 1-phosphate synthase cosuppression sequence thereby yielding a reduced amount of myo-inositol 1-phosphate synthase and consequently reduced phytate in said

Method for reducing phytate in canola meal using genetic manipulation involving myo-inositol 1-phospathe synthase gene

Genetic constructs, transformation vectors and methods are taught for production of transgenic plants which can be selectively removed from a growing site by application of a chemical agent or physiological stress. The invention links a target gene for the trait of commercial interest to a conditionally lethal gene, which can be selectively expressed to cause plant death. By use of the genetic constructs, transformation vectors and methods of the present invention, invasion of environments and contamination of commercial non-engineered productions by transgenic plants can be avoided. Methods are also taught for transformation of Brassica species.

The present invention provides a method of making a genetically transformed plant which has an altered content of at least one product of a secondary metabolic pathway. The method consists of introducing into a plant cell capable of being transformed and regenerated to a whole plant a DNA expression cassette. The expression cassette includes DNA sequences required for transformation and selection in plant cells. It also includes a DNA sequence that, under the control of a promoter active in plant cells, encodes a protein capable of modifying the utilization of a substrate in the secondary metabolic pathway. The substrate is not a primary metabolite of the group selected from glucose, amino acids, common fatty acids and nucleotides. A plant or plant tissues including seeds can then be recovered having an altered content of at least one product of the secondary metabolic pathway. The invention also provides for feed products derived from the plants and seeds obtained according to the method.

Method and compositions for modifying levels of secondary metabolic compounds in plants

The sinapine content of seeds of , and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H:) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.

Transgenic reduction of sinapine in crucifera

Modification of plant lipids and seed oils utilizing yeast SLC genes

Growing community of inventors

Saskatoon, Canada

Wilfred A Keller