Lonza Inc.

Evologic Technologies Gmbh

Wieland Reichelt

Christoph Herwig

Patrick Sagmeister

Matthias Funke

Julian Kager

Paul Krautwaschl

Markus Brillmann

The present invention relates to bioreactors for root organ cultures that enable large parts of the inoculation or biomass transfer, cultivation and harvest of the cultures to be performed outside of an aseptic environment. The present invention further relates to methods of producing and aseptically harvesting roots, and methods of producing root cultures using the aforementioned bioreactors alone or as part of an apparatus further comprising an inoculation vessel or at least one further bioreactor.

Bioreactors for root organ cultures

A method for producing a recombinant protein of interest (POI) is provided, comprising the steps of (a) culturing cells in a cell culture medium to express said POI by adding a feed comprising at least one substrate to said cell culture, (b) applying a feeding strategy based on calculating, setting and optionally controlling the specific substrate uptake rate qof the cells during the induction phase and/or production phase of the POI, wherein qis set to be close to the maintenance rate of the cell culture; and (c) isolating said POI from the cell culture.

Fermentation process

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Vienna, Austria

Wieland Reichelt