Mwi, Inc.

Sequoia-turner Corporation, a Corp. of Ca

Wieland E Von Behrens

Jerry B West

Mervin L Gangstead

James R Boyd

Roger Bourree

George Chappell

James G Young

Jean-Charles Pina

John Glazier

Sherry Haiflich

Bruce A Director

John M Roche

The present invention relates to an optical system for an apparatus for multi-part differential particle discrimination to facilitate analysis, classification, and sorting of various fluid components for presentation. The optical system is characterized by one or more of the following: a synchronized illumination beam and flow cell conduit, a flow cell arrangement to control back reflection, and light sensor arrangement to particularly gather a specific range of light scatter, such specific range of light scatter directly corresponding to at least one type of particle capable of being identified by the apparatus.

Optical device and method for multi-angle laser light scatter

Optical system for multi-part differential particle discrimination and an apparatus using the same

An apparatus for analyzing particulates including solutes in a sample liquid suspension in which the sample liquid suspension flows through an aperture into a first region of a reservoir containing particle free liquid to form a jet which entrains particle free liquid to ensheathe the jet. The jet passes through a muffler which includes a funnel and a throat into a second region of said reservoir where the particle free liquid and particulates are mixed. The throat is configured to prevent regurgitation of liquid from the second region back into the first region. A return passage connects the second region of the reservoir to the first region. The energy of the jet stream causes liquid to flow from the second region to the first region to provide particle free liquid to the first region. The return passage is configured or contains means to continuously mix the advancing liquid to prevent central streamers and the peripheral Coanda effect. This assures that during particle analysis only clean liquid reaches the first region, and that during cleanout particle free liquid admitted into the first region displaces all analyzed sample liquid without carryover.

Method and apparatus for analyzing particulate matter

A method for identifying, characterizing, categorizing and enumerating cells within a cell population. The survivorship characteristics of the different cell populations is used to obtain quantitative and qualitative information about the cell populations initially present in the sample solution before conditions were imposed on the sample solution to elicit a response. The monitored cell survivorship response may be either direct disappearance of intact cells or the appearance of cell structures, carcasses, ghosts or residuum. In a preferred embodiment, a leukocyte cell decay rate in the presence of an erythrolytic agent is determined by monitoring leukocyte counts at several time intervals after the addition of the erythrolytic agent to the sample solution. The leukocyte decay rate is then used to indicate the presence of a fragile leukocyte population, and to accurately estimate the number of leukocytes initially present in the whole blood sample. The use of stronger erythrolytic agents in samples with lyse-resistant erythrocytes while preserving the accuracy of leukocyte counts is allowed. The present method permits calculation of a leukocyte decay rate which can be back extrapolated to time zero to provide an accurate estimate of the leukocyte count initially present in the whole blood sample.

Method for accurately enumerating and sensitively qualifying

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Hillsborough, CA, United States of America

Wieland E Von Behrens