Bayer Aktiengesellschaft

Werner Schroder

Eugen Schnabel

Gerd Reinhardt

Wolfgang Bruns

Ernst-August Auerswald

Dietrich Horlein

Michael Kotick

Hans-Georg Dellweg

Wolfgang Piepersberg

Jurgen Georg Lenz

Ansgar Stratmann

Jurgen Distler

Anneliese Crueger

Michael Hemker

Beate Schaper

Klaus Goeke

Hermann Pape

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

Processes for preparing acarviosyl transferase and for using it in the

Peptides having essentially the sequence of bovine pancreatic trypsin inhibitor (aprotinin) wherein one or more of the amino acids at positions 15, 16, 17, 18, 34, 39 and 52 are replaced by any naturally occurring amino acid produced by recombinant DNA technology, process, expression vector and recominant host therefor and pharmaceutical use thereof. Such peptides being useful as therapeutic agents in diseases connected with the presence of excessive amounts of proteinases.

Variants of bovine pancreatic trypsin inhibitor produced by recombinant

Variants of bovine pancreatic trypsin inhibitor and pharmaceutical use

Microbially produced aprotinin and aprotinin homologs used for treating patients suffering from an excess release of pancreatic elastase, serum elastase or leukocyte elastase.

Homologs of aprotinin produced from a recombinant host, process,

Homologs of aprotinin produced from a recombinant host, process

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Wuppertal, Germany

Werner Schroder