Boehringer Mannheim Gmbh

Werner Naser

Erasmus Huber

Christa Hubner-Parajsz

Ulrich Essig

Brigitte Drager

Helmut Lill

Beatus Ofenloch-Hahnle

Andreas Dessauer

For the qualitative or/and quantitative detection of a substance to be determined in a test sample with the aid of an immunoassay or nucleic acid hybridization assay the following components are used: a) a capture reagent which enables a specific detection of the substance to be determined by means of two different binding sites 1) if desired together with further test components and 2) is bound or is capable of binding to an active solid phase via a specific binding pair one partner of which is linked to the capture reagent and the second partner of which is coupled to an active solid phase b) an active solid phase and c) an inactive solid phase which substantially corresponds to the active solid phase but to which the capture reagent cannot bind, wherein the test sample is either firstly brought into contact with the inactive solid phase alone and only later with the active solid phase, or is simultaneously brought into contact with the active and inactive solid phase during which the capture reagent and if desired further test components are either present from the start or are only added later and the substance to be determined is detected.

Method for the qualitative or/and quantitative detection of an analyte

A method for determining collagen degradation products in a body fluid sample using a non-competitive or competitive immunoassay is disclosed, the method comprising incubating the body fluid sample with an amount of antibody which specifically reacts with the amino acid sequence of SEQ ID NO. 2 of collagen l or a fragment thereof, and determining the collagen degradation products bound to the amount of the antibody. A kit for such a method is also disclosed.

Immunoassay for the detection of collagen I

Disclosed are antibodies which specifically bind the amino acid sequence of SEQ ID NO. 1 of collagen I. A hybridoma cell line which produces the antibodies is also disclosed.

Hybridoma for producing antibody for collagen I

The present invention concerns an improved method for the determination of fibrin in body fluids in which before the actual determination the sample solution containing fibrin is incubated in the presence of thiocyanate, iodide, magnesium or/and guanidinium ions.

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Weilheim, Germany

Werner Naser