Merck Patent Gesellschaft MIT Beschrankter Haftung

Schering Aktiengesellschaft

Boehringer Mannheim Gmbh

N.v. Nutricia

Merck Patent Gesellschaft MIT

Boehringer Amnnheim Gmbh

Werner Goebel

Siegfried Neumann

Peter Schubert

Andreas Bubert

Christa Burger

Winfried Linxweiler

Servatius H Notermans

Martina Pawelzik

Trinad Chakraborty

Bernd Stahl

Joachim J Schmitt

Berndt Finke

Hans-Joachim Mollenkopf

Albrecht Ludwig

Monika Goetz

Gottfried Hofmann

Stefan Kohler

Jörg Slaghuis

Stefan Köhler

Fred Heffron

Stephen J Libby

Neutral straight-chain or branched oligosaccharides for preventing the invasion and infection of mammal cells by pathogens and for fighting diseases caused by such pathogens are disclosed. Food, dietetic products and pharmaceutical agents containing oligosaccharides consist of a base unit of formula (I), and between 0 and 19 other units of formula (II), which are linked directly or indirectly to the base unit. Gal represents a galactose monosaccharide unit; Glc represents a glucose monosaccharide unit; HexNAc rpresents an N-acetylated galactosamine or glucosamine monosaccharide unit (GalNAc or GlcNAc); R independently represent a &#x3b2; 1-3 or &#x3b2; 1-6 glycosidic link to HexNAc monosaccharide of the next [Gal-HexNAc] unit (II), and on a terminal [Gal-HexNAc] unit, or R are not present; and a terminal [Gal-HexNAc] unit, wherein R represent a deoxyhexose radical, can have another deoxyhexose radical on the HexNAc monosaccharide unit. 

Anti-infectious carbohydrates

The invention relates to agents and processes for detecting bacteria of the genusin particularThe agents according to the invention include primers whose sequence is selected from the iap gene ofIn addition, the agents according to the invention include peptides whose sequence is selected from the p60 protein and which are suitable for producing specific antibodies for the immunological detection of

Processes and agents for detecting Listerias

The invention relates to the introduction of DNA or RNA sequences into a mammalian cell to achieve controlled expression of a polypeptide. It is therefore useful in gene therapy, vaccination, and any therapeutic situation in which a polypeptide should be administered to a host or cells of said host, as well as for the production of polypeptides by mammalian cells, e.g., in culture or in transgenic animals.

Delivery of polypeptide-encoding plasmid DNA into the cytosol of

The invention relates to agents and processes for detecting bacteria of the genus Listeria, in particular L. monocytogenes. The agents according to the invention include primers whose sequence is selected from the iap gene of L. monocytogenes. In addition, the agents according to the invention include peptides whose sequence is selected from the p60 protein and which are suitable for producing specific antibodies for the immunological detection of L. monocytogenes.

Processes and agents for detecting listerias

The invention relates to agents and methods for the immunological detection of bacteria of the genus Salmonella. The agents according to the invention include peptides whose sequence is selected from the PagC protein, and which are suitable for producing specific antibodies for the immunological detection of salmonellae.

Antibodies directed to pagC peptides for the detection of salmonellae

The invention relates to oligonucleotides which are suitable for use as nucleic acid probes or as primers for the detection of Enterobacteriaceae, in particular of pathogenic Enterobacteriaceae, such as, for example, Salmonella sp. The oligonucieotides according to the invention are selected from the salmolysin-like DNA region and possess a sequence according to one of the formulae IIIa to IIIh, or a corresponding complementary sequence:

Oligonucleotides for the detection of enterobacteriaceae selected from

The present invention is directed to Listeria monocytogenes specific protein that is encoded by nucleotide sequence of FIG. 1, the complementary sequence of which hybridizes to the nucleotide sequence of FIG. 1 at 5-6x SSC and 42.degree.-60.degree. C. A Listeria monocytogenes specific protein is also described having an amino aicd sequence as set forth in FIGS. 1A-1D. The proteins are suitable for the production of antibodies against Listeria monocytogenes.

Listeria monocytogenes specific proteins, and uses thereof

Nucleic acids which preferably hybridize under the hybridization conditions 5-6.times. SSC and 42.degree. C. to 60.degree. C. with a 2.5 kb large KpnI-BamHI fragment from pLM63, preferably with a nucleic acid according to FIG. 1. Such nucleic acids are suitable for hybridization tests for Listeria bacteria if they contain reporter groups. Proteins are also described which are suitable for the production of antibodies against Listeria bacteria.

Method for the determination of pathogenic listeria bacteria

A Salmonella gene, encoding a cytolysin, has been identified by screening for hemolysis on blood agar. The gene (slyA) is present in every strain of Salmonella examined in Shigella, and enteroinvasive Escherichia coli (EIEC) but not in other enterobacteriaceae. It is encoded near 28.5 minutes on the chromosome. A SlyA (salmolysin) has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. LD.sub.50 and infection kinetics data in mice indicate that the toxin is required for virulence and facilitates Salmonella survival within peritoneal macrophages.

Cytolysin gene and gene product

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Veitshochheim, Germany

Werner Goebel