Micronas Gmbh

Micronas Intermetall Gmbh

Werner Baumann

Mirko Lehmann

Ralf Ehret

Ulrich K Sieben

Bernhard Wolf

Ingo Freund

G&uuml;nter Igel

Martin Brischwein

Hans-J&uuml;rgen Gahle

Günter Igel

Hans-Jurgen Gahle

Hans-Jürgen Gahle

Hans-J&ouml;rg Gahle

Gerd Sulz

Alfred Dumbs

A device for investigating activities of cell cultures in a liquid culture medium includes a receptacle for the culture medium, and one or more sensors for measurement of cell culture activities. An opening for adding and removing liquid culture medium is provided. A separating element can be placed close to the receptacle bottom, forming a partial space with a small volume relative to the entire volume. A flow channel that communicates with the small volume and the reservoir and/or the sensors are placed in the small volume partial space. By adjustment of the separating element distance from the receptacle bottom, a microreaction volume exactly fitting the current requirements is obtained. For regeneration of the cell culture solution in the small volume partial space, the separating element can be elevated and depressed, or liquid can be injected into the small volume by the through-flow channel, providing convective mixing of fresh and spent medium.

Device for performing analysis on cell cultures

A process is provided for the intracellular manipulation of a biological cell () which is positioned adhering to a support area () in a culture medium (). Inside the support area () for the cell () an opening into the membrane of the cell () is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (), thus seals off the cell fluid situated in the interior of the cell () from the culture medium () and insulates the cell fluid against the culture medium (). The interior of the cell () is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier () with a support area () for adhering the cell and a poration tool () for creating the opening in the cell membrane. The poration tool () may be any of various chemical, mechanical and/or electrical devices.

Apparatus for intracellular manipulation of a biological cell

The invention relates to a device for measuring living cells or similar biocomponents comprising a field effect transistor which is provided with a source, a drain and a channel area placed on a substrate. Said channel area connects said source and drain and is provided with a gate-electrode mounted thereon. The gate electrode has at least two laterally disposed parallel electrode areas which are perpendicular to a direction in which the channel area connects the source to the drain in such a way that they are distant and electrically insulated from each other.

Field effect transistor for measuring biocomponents

In a method for examination of the surface of an object for a topographic and/or a chemical property, the object-surface is impinged with surface-structure selective biocomponents for examination of a topographic property and/or with chemoselective biocomponents for the examination of a chemical property, together with a nutrient medium and/or an osmotic protective medium for the biocomponents. The biocomponents contained in the nutrient medium and/or the osmotic protective medium are in contact with the object-surface or are spaced from the object surface by less than the detection range of the biocomponents. The object surface is then examined with the biocomponents contained in the nutrient medium and/or the osmotic protective medium by determining at least one examination measurement value. The examination measurement value is compared with a reference measurement value, and conclusions can be drawn about the topographic and/or chemical properties of the object from the result of the comparison. Using the method, an object surface can be examined for a topographic and/or chemical property in a simple manner with a high measuring sensitivity.

Method for examination of a surface layer

A method is provided for measuring a state variable of a biological cell ( ) located in a nutrient medium ( ) and supported on and adhering to a support area ( ). Within the support area ( ) for the cell ( ) and at a distance from the support area edge, an opening is made in the membrane of the cell ( ). The edge of the cell membrane that surrounds the opening and adheres to the support area ( ) seals off the liquid found inside the cell ( ) from the nutrient medium ( ). Through the opening the state variable ( ) is measured. An apparatus for performing the method is also provided.

Method for measuring a state variable

A process is provided for the intracellular manipulation of a biological cell ( ) which is positioned adhering to a support area ( ) in a culture medium ( ). Inside the support area ( ) for the cell ( ) an opening into the membrane of the cell ( ) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area ( ), thus seals off the cell fluid situated in the interior of the cell ( ) from the culture medium ( ) and insulates the cell fluid against the culture medium ( ). The interior of the cell ( ) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier ( ) with a support area ( ) for adhering the cell and a poration tool ( ) for creating the opening in the cell membrane. The poration tool ( ) may be any of various chemical, mechanical and/or electrical devices.

Method for intracellular manipulation of a biological cell

Method and apparatus for measuring a state variable

A chip arrangement (,) has a substrate board (,) with an opening (,), into which a carrier chip (,) is inserted, which has an electrical or electronic structural component (,). At least one conductor path (,) is integrated into the carrier chip (,), which connects the structural component (,) to the electrical connection contact (,). The carrier chip (,) is inserted into the opening (,) in such a way that its ends project beyond the opposite-facing, flat-sided surfaces (,&prime;) of the substrate board (,), and thereby form overhangs (,&prime;). Here, the structural component is arranged on the overhang (,) projecting beyond the one surface (,), and the connection contact (,) is arranged on the overhang (,&prime;) projecting beyond the other surface (,&prime;), and the conductor path (,) connecting the structural component (,) and the connection contact (,) passes through the opening (,). A seal is arranged between the substrate board (,) and the carrier chip (,).

Chip arrangement

A measuring device (,) for measuring or investigating physiological parameters using biological cells or chemical and biologically active substances contained in an analyte (,). The measuring device comprises a sensor (,) with an electronic measurement structure (,) located on a substrate (,). Function-specific receptor cells and/or target cells (,) form part of the sensor (,) and are in direct contact with the measurement structure (,). For measurement, the medium under investigation can be brought into contact with the target cells or receptor cells (,). The measuring device (,) is especially compact in its design and facilitates largely feedback-free investigation of biological or chemical components contained in the analyte.

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Bühl, Germany

Werner Baumann