Hoechst Aktiengesellschaft

Aventis Pharma Deutschland, Gmbh

Werner Aretz

Klaus Sauber

Gerhard Seibert

Dirk Bottger

Peter Welzel

Alois Tumulka

Kurt Hobert

Ulrich Gebert

Hans-Wolfram Fehlhaber

Harald Furrer

Heinz-Joachim Hinze

Laszlo Vertesy

Bimal N Ganguli

Eberhard Ehlers

Hans-Harald Sedlacek

Paul Habermann

Michael Kurz

Hans P Kraemer

Gunther J Riess

Klaus-Peter Koller

Hans G Berscheid

Johann Then

Heinrich Decker

Gerhard Huber

Julia Gandhi

Laszl&oacute; V&eacute;rtesy

Werner Badziong

Ulrich Holst

Gauknapalli C Reddy

Ratan S Sood

Frank Rainer Schmidt

Udo Hedtmann

Klaus Peter Koller

Philipp Willems

Joerg Moeller

Calcium salts of lipopeptide antibiotics, method for producing same and their use

The invention relates to a novel process for using the yeast ADH II promoter system for the production of heterologous proteins in high yields, for example for preparing hirudins, miniproinsulins, leptins or derivatives thereof.

Process for using the yeast ADH II promoter system for the production of

The gene encoding glutarylacylase (GA) contained in the plasmid pCM 145 (DSM 6409) permits the expression of GA in E. coli in high yields.

Process for the preparation of glutarylacylase in large quantities

Improved, induction-free process for the recombinant preparation of glutarylamidase. The invention relates to a recombinant process for preparing glutarylamidase (GA), wherein the GA gene is expressed constitutively from an expression vector. In certain embodiments, the promoter for constitutively expressing the GA gene is a trc promoter or a trc-equivalent promoter which does not have any repressor or has a non-functional repressor.

Induction-free process for the recombinant preparation of glutarylamidase

The present invention relates to a growth-coupled fed-batch fermentation of oxygen-dependent microorganisms. It has been found that oxygen-dependent microorganisms can be advantageously fermented if the carbon supply is carried out as a function of the oxygen uptake rate.

Oxygen-dependent fermentation of microorganisms

A Rhodotorula rubra strain has been found which reduces pentoxifylline to 100% to give the S-alcohol. Moreover, other oxoalkylxanthine derivatives can also be convened into the corresponding S-alcohol. The microbiologically obtained S-(+)-enantiomers can then be converted stereoselectively into the respective R-(-)-enantiomers. The corresponding S-alcohols and the R-alcohols obtained by enantioselective inversion of configuration cause an increase in cerebral blood flow.

7-methoxymethly-3-methyl-1-(5-oxohexyl)xanthine

L-2-Amino-4-methylphosphinobutyric acid can be prepared by a racemate resolution in the presence of D-aminoacid and L-aminoacid transaminases by reaction with .alpha.-keto acids and amino-group donors. In particular, transaminases from Bacillus licheniformis and from Escherichia coli are used.

Process for the preparation of L-phosphinothricin using transaminases of

An esterase with a molecular weight of 55,200 .+-.300 Da is suitable for the chemoselective conversion of a compound of the formula II into a compound of the formula I. ##STR1##

7-amino-3-methoxymethyl-3-cephem-4-carboxylic ester hydrolase, process

The novel substances nordanoxamine and desferrinordanoxamine, and desferrisalmycin A, B, C and D, as well as the known substances danoxamine and desferridanoxamine, are prepared from the antibiotics salmycin A, B, C and D, which are obtained by fermentation. These substances are used as chelating agents and pharmaceuticals.

Chelating agents, their preparation from the antibiotics salmycin A,B,C,

A new Bacillus species and the appropriate enzymes obtained therefrom can be used to break down phosphoglycolipid antibiotics. The breakdown products of moenomycins display antibiotic activity or can be used as building blocks for the synthetic preparation of transglycosylase inhibitors.

Bacillus strain for breaking down moenomycins

Salmycins, a process for their preparation and their use as a pharmaceutical Compounds of formula I, ##STR1## wherein R denotes .dbd.NOH (a), .dbd.O (b); --OH and --H (c), --NH.sub.2 and --H (d) or .dbd.N--O--R'(e);

Salmycins, a process for their preparation and their use as a

A Rhodotorula rubra strain has been found which reduces pentoxifylline to 100% to give the S-alcohol. Moreover, other oxoalkylxanthine derivatives can also be converted into the corresponding S-alcohol. The microbiologically obtained S-(+)-enantiomers can then be converted stereo-selectively into the respective R-(-)-enantiomers. The corresponding S-alcohols and the R-alcohols obtained by enantioselective inversion of configuration cause an increase in cerebral blood flow.

Rhodotorula rubra useful in a process for the preparation of

The invention relates to a process for the preparation of MA, which ensures MA production by enzymatic catalysis in glycine/NaOH buffer, and by the use of a phosphate-containing culture medium for Bacillus sp. DSM 4675 and by extraction of the biomass with acetone.

Process for the preparation of MA

A new microorganism for breaking down moenomycins, a process for the breakdown, and the use of the breakdown products are disclosed. A new Bacillus species and the appropriate enzymes obtained therefrom can be used to break down phosphoglycolipid antibiotics. The breakdown products of moenomycins display antibiotic activity or can be used as building blocks for the synthetic preparation of transglycosylase inhibitors.

Microorganism for breaking down moenomycins, a process for the

A Rhodotorula rubra strain has been found which reduces pentoxifylline to 100% to give the S-alcohol. Moreover, other oxoalkylxanthine derivatives can also be converted into the corresponding S-alcohol. The microbiologically obtained S-(+)-enantiomers can then be converted stereoselectively into the respective R-(-)-enantiomers. The corresponding S-alcohols and the R-alcohols obtained by enantioselective inversion of configuration cause an increase in cerebral blood flow.

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Konigstein/Taunus, Germany

Werner Aretz