Grail, Inc.

Wenying Pan

Matthew H Larson

Arash Jamshidi

Xiao Yang

Hyunsung John Kim

H John Kim

Pranav Parmjit Singh

Eric Michael Scott

Mohini Jangi Desai

Methods for measuring subpopulations of ribonucleic acid (RNA) molecules are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Methods for detecting disease using analysis of RNA

Cell free nucleic acids from a test sample obtained from an individual are analyzed to identify possible fusion events. Cell free nucleic acids are sequenced and processed to generate fragments. Fragments are decomposed into kmers and the kmers are either analyzed de novo or compared to targeted nucleic acid sequences that are known to be associated with fusion gene pairs of interest. Thus, kmers that may have originated from a fusion event can be identified. These kmers are consolidated to generate gene ranges from various genes that match sequences in the fragment. A candidate fusion event can be called given the spanning of one or more gene ranges across the fragment.

Alignment free filtering for identifying fusions

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Menlo Park, CA, United States of America

Wenying Pan