Mgi Tech Co., Ltd.

Bgi Shenzhen

Shenzhen Technology University

Complete Genomics, Inc.

Bgi-shenzhen Co., Limited

Qingdao Mgi Tech Co., Ltd

Bgi Shenzhen & Bgi Shenzhen Co., Limited

Egi Tech (shen Zhen) Co., Limited

Mgi Tegh Go., Ltd.

Wenwei Zhang

Jun Wang

Hui Fu Jiang

Yuan Jiang

Chunyu Geng

Radoje Drmanac

Xia Zhao

Chongjun Xu

Ao Chen

Yuliang Dong

Xun Xu

Snezana Drmanac

Qiaoling Li

Erkai Liu

Bo Wang

Jin Yang

Hui Xing Wang

Lin Wang

Andrei Alexeev

Xing Liu

Xi Zhang

Wei Chen

Zhenhua Zhang

Jian Wang

Lingyu He

Li Chen

Jing Guo

Lin He

Hanjie Shen

Lin Li

Dong Li

Handong Li

Yuan Li

Bin Xie

Tengyue Li

Dennis G Ballinger

Zhaohui Wang

Tong Wang

Mei Li

Meihua Gong

Aoli Wang

Yue Zheng

Kai Tian

He Zhang

Yingxin Zhang

Yuning Zhang

Yanyan Zhang

Ming Ni

Yue Shen

Zhougang Zhang

Huaiqian Xu

Yujun Zhou

Xiaojun Ji

Rongrong Guo

Sha Liao

Shengmao Liu

Fen Liu

Weimao Wang

Huanhuan Liu

Hongyan Han

Shujin Fu

Jinpeng Tian

Yaqiao Li

Lulu Zha

Zhijian Lv

Jing Yu

Yao Wang

Chao Dong

Mengzhe Shen

Hui Wang

Ou Wang

Xiaofang Cheng

Ruoying Chen

Junqiang Xu

Quanxin Yun

Evan Hurowitz

Yang Xi

Pengjuan Liu

Yongwei Zhang

Zhuokun Li

Shengming Zhao

Fei Fan

Luman Cui

Y Tom Tang

Guangyi Fan

Kailong Ma

Zixin Lin

Zihao Jiao

Gan Ma

Guangying Guo

Zhiming Chen

Ziqi Xu

Xiaogang Song

Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Rolling circle amplification method, method for preparing sequencing library, and DNA nanosphere prepared therefrom

Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Method for sequencing long-fragment nucleic acid

The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.

Method for sequencing polynucleotides

Sequencing instrument

Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S).

Magnetic bead-based detection method, storage medium, and detection device

Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).

Recombinant KOD polymerase

Provided is an underwater wireless optical communication system. The system includes an emitting end and a receiving end; where the emitting end includes a beam emitting module and an optical phased array, where the beam emitting module is configured to emit a communication beam obtained by modulating communication information, and the optical phased array is configured to adjust an emission direction of the communication beam; and the receiving end includes a tracking module and an information demodulation module, where the tracking module is configured to receive a target communication beam emitted after adjustment by the optical phased array and align the target communication beam, and the information demodulation module is configured to demodulate the received target communication beam to obtain the communication information.

Underwater wireless optical communication system

Provided is a visible light communication system. The system includes a modulation and light emission module configured to emit a communication light ray obtained by modulating information to be communicated; and a tracking and light sensing module configured to receive and demodulate the communication light ray to obtain the information to be communicated and further configured to align the tracking and light sensing module with the communication light ray according to the angle between the tracking and light sensing module and the communication light ray.

Visible light communication system

A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

Nucleic acid probe and nucleic acid sequencing method

A ground simulation device and method for an on-orbit manipulation of a space manipulator is provided. The ground simulation device includes: a dual-arm robot, configured to simulate the space manipulator operating a target object; a suspension device, including a fixed post and passive rods, where the passive rods are movably connected with a top end of the fixed post, and the target object is suspended to the passive rods; and a simulation platform, configured to fix the dual-arm robot and the suspension device thereon. The ground simulation device provides the passive rods on the suspension device and suspends the target object to the passive rods, thus overcoming the gravity of the target object. In addition, the passive rods can drive the target object to move under an influence of an external force, achieving a similar suspension effect to that in space, and providing a desired, safe, and reliable implementation effect.

Ground simulation device and method for on-orbit manipulation of space manipulator

Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element

A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and () obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Method for obtaining single-cell mRNA sequence

The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

Nucleic acid sequencing method

Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.

Hooked probe, method for ligating nucleic acid and method for constructing sequencing library

Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3' end, a polymerase, a reagent 1 for excising the blocking group attached to the 3&#x2032; end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.

Nucleic acid sequencing method and nucleic acid sequencing kit

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Shenzhen, China

Wenwei Zhang