Jiangsu University

Wenting Li

Xiaobo Zou

Jiyong Shi

Xiaowei Huang

Zhihua Li

Xuetao Hu

Zhiming Guo

Xinai Zhang

Junjun Zhang

Yongqiang Shi

Haijun Shi

Xueping Cui

Yahui Li

Nini Liang

Pei Jiang

The present invention belongs to the field of organophosphorus pesticide (OP) detection, and relates to a method for detecting an OP by a microfluidic chip based on a fluorescent sensing film. A porous fluorescent sensing film and the microfluidic chip are first constructed. The fluorescent sensing film is fabricated through layer-by-layer self-assembly of a platinum nanoparticle@oxalate-metal-organic framework (MOF) composite and a porous two-dimensional (2D) nanosheet, and has the functions of specifically detecting OPs and blocking macromolecular interferents. The microfluidic chip includes a sample channel, injection channels, reaction tanks, microfluidic channels, a detection tank, and an optical fiber channel, such that sample pretreatment and detection processes are integrated in the chip. An OP detection system is established by combining a portable constant-pressure syringe pump, a laser, a spectrometer, a signal transmitter, and a signal indicator, such that test devices are miniaturized and integrated and the OP detection is standardized.

Method for detecting organophosphorus pesticide by microfluidic chip based on fluorescent sensing film

Fluorescent test paper for detecting mercury ions is firstly prepared, the fluorescent test paper including a fluorescence development area and a standard fluorescent colorimetric card. With reference to an excited color corresponding to a reference channel in the standard colorimetric card, the color corresponding to a carbon quantum dots distribution area on the fluorescent test paper is observed to check the fluorescence detection environment during detection with the fluorescent test paper. In colorimetric detection of the content of mercury ions, the concentration of Hgis detected based on an excited color, in the standard colorimetric card, that corresponds to a standard mercury ion sample and is closest to a color signal in a copper nanoclusters distribution area on the fluorescent test paper.

Fluorescent test paper for detecting mercury ions and detection method using the same

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Jiangsu, China

Wenting Li