Peking University

Hygea Medical Technology Co., Ltd.

Edigene Biotechnology Inc.

Edigene Therapeutics (beijing) Inc.

Edigene Inc.

Edigene Therapeutics Inc.

Wensheng Wei

Pengfei Yuan

Zongyi Yi

Liang Qu

Chunhui Wang

Shiyou Zhu

Zhuo Zhou

Yuan Zhang

Yuan Liu

Feng Tian

Jian Xiao

Chengqi Yi

Chenxu Zhu

Jingjing Yang

Yanxia Zhao

Yuexin Zhou

Nengyin Liu

Gangbin Tang

Yiou Chen

Zhongzheng Cao

Hongmin Zhang

Zexuan Yi

Shengjie Guo

Bowei Chen

Cuihu Liu

Fuliang Luo

Lulu Liu

A high and low temperature composite ablation surgery system, relating to the technical field of cryotherapy, used to improve the safety and effectiveness of therapy, and comprising a main unit () and a cryogenic-thermal ablation probe () connected to the main unit (). The main unit () comprises a cold working medium supply system (), a hot working medium supply system (), and a working medium distribution system (). The working medium distribution system () can respectively control the cold working medium supply system () to deliver a cold working medium to the cryogenic-thermal ablation probe () and control the hot working medium supply system () to deliver a hot working medium to the cryogenic-thermal ablation probe (). Therefore, after low-temperature therapy is completed, a therapy area can be quickly rewarmed, thereby providing a basis for improving the safety, economy and convenience of the surgery.

High and low temperature composite ablation surgery system

RVDs with recognition preferences for 5mC, 5hmC and 6 mA and different binding properties to these epigenetic modifications are identified in this present invention. Methylation-dependent gene activation, efficient genome editing, targeted detection of 5hmC and other applications can be achieved by using these RVDs. The present invention therefore provides an isolated DNA binding polypeptide containing TALEs, a fusion protein, a polynucleotide, a vector comprising the polynucleotide and a host cell, and the use of the protein comprising TALE repeats domain in the preparation of a reagent for detecting a methylated base in a target sequence of a gene of interest, as well as a method for targeting and binding to a target sequence of a gene of interest in a cell.

Tale RVD specifically recognizing DNA base modified by methylation and application thereof

The present application provides methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA. The present application further provides deaminase-recruiting RNAs used in the RNA editing methods and compositions comprising the same.

Methods and compositions for editing RNAs

Provided are methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA, deaminase-recruiting RNAs used in the RNA editing methods, compositions and kits comprising the same.

Targeted RNA editing by leveraging endogenous ADAR using engineered RNAs

A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.

Gene knockout method

Growing community of inventors

Beijing, China

Wensheng Wei