Nanoprobes, Inc.

Wenqiu Liu

James F Hainfeld

Richard D Powell

Vishwas N Joshi

The present invention provides compositions, kits, assemblies of articles and methodology for carrying out processes that permit biological enzymes to act directly on metals and metal particles. In particular, the invention concerns using enzymes to selectively deposit metal to the vicinity of a target molecule. The invention also relates to linking of metals to enzyme substrates, control of enzymatic metal deposition and applications of enzymatic metal deposition to sensitively and selectively detect target molecules such as biomarkers in various biological samples, such as chromogenic immunohistochemical (IHC) detection in situ by using microscopy.

Kit for enzymatic deposition of a metal

Disclosed embodiments concern site-specific, enzymatic-directed deposition of elemental metal for in-situ analysis. Enzyme substrates are contacted with metal ions and subsequently direct the deposition of elemental metals. Sensitive and selective detection of target molecules, such as biomarkers in various biological samples, can be obtained using various methods, such as in-situ chromogenic immunohistochemical (IHC) detection with bright field light microscopy.

Method for detecting a target using enzyme directed deposition of elemental metal

The present invention provides methods for the use of enzymes to selectively deposit metal to the vicinity of a target molecule. The invention also relates to applications of enzymatic metal deposition to sensitively and selectively detect target molecules such as biomarkers in various biological samples, such as chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope.

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Dix Hills, NY, United States of America

Wenqiu Liu