Reynolds Technologies, Inc.

R.j. Reynolds Tobacco Company

Other

Wennuan Liu

Michael Francis Dube

Luis Mayan Dominguez

Jerry Wayne Lawson

Thomas A Perfetti

Paul Andrew Brinkley

William Monroe Coleman, Iii

Marvin Glenn Riddick

Gary Mark Hellmann

Watson Minyard Dufour

Richard Carlton Reich

Stephen Keith Cole

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generateplant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improvedplant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

Tobacco having modified nicotiana tobacum

Tobacco having increased threonine

A method for chemically modifying a tobacco material during a curing process involves treating the tobacco material in a curing enclosure at a temperature and for a time sufficient to produce cured tobacco. The method also involves contacting the tobacco material with a chemical reagent before and/or during the time period that the tobacco material is cured so that the chemical reagent can interact with the tobacco material, thus resulting in further change in the chemical nature of the cured tobacco material. An exemplary chemical reagent is an ammonia source, which can be applied to a tobacco material in a variety of ways, including by spraying an aqueous ammonia source onto the tobacco or by introducing gaseous ammonia into the curing enclosure during the curing process.

Method for chemically modifying tobacco during curing

[object Object]

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

High threonine producing lines of Nicotiana tobacum and methods for producing

Salvia is regenerated via organogenesis using plant tissue culture techniques in a multistage culturing process. Roots can be induced from regenerated shoots, and the regenerated plants can be transferred to soil for further growth after the root system is well established.

Method for regeneration of salvia species

Growing community of inventors

Clemmons, NC, United States of America

Wennuan Liu