Syngenta Participations Ag

Wenling Wang

Qiudeng Que

Mary-Dell Matchett Chilton

Mark Scott Rose

Heng Zhong

Weining Gu

Zhongying Chen

Ailing Zhou

Yinping Lucy Qin

Kimberly Ann White

Mary Fielder

Yanshan Ji

Michele Susan Yarnall

Karen Moore

Jamie Huang

Rachel Whinna

Er-Te Jamie Huang

Karen Elizabeth Moore

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Targeted insertion sites in the maize genome

The present invention provides a method of isolating nucleic acid and protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample.

Methods and compositions for dual extraction of protein and nucleic acid

Growing community of inventors

Durham, NC, United States of America

Wenling Wang