University of California

Wenjun Du

Jacquelyn Gervay-Hague

Matthew Schombs

Suvarn Subhash Kulkarni

Sally Denardo

Arutselvan Natarajan

The present invention provides a one-pot method of preparing an unprotected &#x3b1;-O-glycolipid. The first step involves contacting a protected &#x3b1;-iodo sugar with a catalyst and a lipid comprising a hydroxy group, under conditions sufficient to prepare a protected &#x3b1;-O-glycolipid. The second step involves deprotecting the protected &#x3b1;-O-glycolipid under conditions sufficient to prepare the unprotected &#x3b1;-O-glycolipid, wherein the contacting and deprotecting steps are performed in a single vessel. The present invention also provides a one-pot method of preparing an unprotected &#x3b2;-O-glycolipid following the steps for the preparation of the unprotected &#x3b1;-O-glycolipid.

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The present invention provides for a practical, universal and efficient method to ligate two large macromolecules (e.g., proteins) using the alkyne-azide 1,3-dipolar cycloaddition reaction to produce a conjugated macromolecule, such as a multivalent scFv. The present invention also provides for conjugate macromolecules comprising a plurality of macromolecule components cross-linked through at least one linking group comprising at least one 1,2,3-triazole moiety, wherein at least 50 percent of the macromolecule components in the conjugate macromolecule has only one site available for cross-linking.

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Mount Pleasant, MI, United States of America

Wenjun Du