The United States of America As Represented by the Administrator of the U.S. Environmental Protection Agency

The United States of America As Represented by the Administrator of the

University of Cincinnati

U.S. Environmental Protection Agency

Wendy Jo Davis-Hoover

Stephen J Vesper

James A Ryan

Harry J Guttman

A bioremediation of subsurface soil formations contaminated with hazardous wastes is achieved by hydraulic fracturing of the subsurface soil formation with simultaneous introduction of sodium percarbonate coated with polyvinylidene chloride as a solid oxygen source (SOS) for establishing colonies of the biodegrading bacteria within the fractures of the soil formation.

Process for the biodegradation of hydrocarbons and ethers in subsurface soil by introduction of a solid oxygen source by hydraulic fracturing

A medium for culturing microorganisms in the presence of Pb uses as a phosphate component, an O-phosphate-L-amino acid, to provide a source of phosphate for the microorganisms so as to avoid precipitating lead.

Medium for cultivating microorganisms

Bioavailability of lead and other heavy metals in the environment may be reduced by addition of microorganisms which sequester lead from the environment in the presence of phosphate. The microorganisms are highly mobile and are, therefore, capable of scavenging a material for lead, which they then sequester. The method basically consists of reducing bioavailability of lead in the environment by addition of,strain CHL004 (ATCC 55937) to said environment in the presence of phosphate which contains at least stoichiometric equivalent amounts of phosphate to lead.

Method for reducing bioavailability of lead by a lead-sequestering soil bacterium

The invention is a consortium of thermophilic methanotrophic organisms in culture medium containing said consortium reproduced at temperatures of 50.degree. C. to 80.degree. C., said consortium being comprised primarily of ovoid or rod-shaped organisms. The consortium can be instilled into soil or water to degrade pollutants, especially hydrocarbons and substituted hydrocarbons.

Growing community of inventors

Wyoming, OH, United States of America

Wendy Jo Davis-Hoover