International Minerals & Chemical Corp.

Pitman-moore, Inc.

Wendy J Cain

Paula M Keith

Matthew D Hilton

Paula Meyers-Keith

A high titer fermentation process using transformed Escherichia coli cells carrying a plasmid containing DNA which codes for Somatomedin C is disclosed. Titers of about 900-1000 mg/L have been obtained using the method of the present invention; about two times the titer of prior art processes.

High titer production of human somatomedin C

A microorganism of the genus Pseudomonas (ATCC 53716) metablizes cinnamate to produce acetophenone in a receoverable quantity upon fermentation in an aqueous nutrient medium containing cinnamate as a carbon source and, optionally, assimilable sources of nitrogen and inorganic substances.

Microorganism ATCC53716 and process for producing acetophenone

A high density fermentation process for high yield production of bovine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for bovine growth hormone under the control of a bacteriophage lambda promoter-operator and a plasmid containing the .lambda.cI857 gene which codes for the temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept at 26.degree.-30.degree. C. Production of bovine growth hormone is then induced by raising the temperature of the medium to at least about 42.degree. C. The temperature is then reduced to about 38.degree. C. to 41.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.

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Terre Haute, IN, United States of America

Wendy J Cain