Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Keysight Technologies, Inc.

University of Tennessee Research Foundation

Huazhong University of Science and Technology

Wuhan University

China University of Geosciences (wuhan)

Oilcrops Research Institute of Chinese Acadamy of Agriculture Sciences

Oil Crops Research Institute, Chinese Acadamy of Agricultural Sciences

Wen Zhang

Peiwu Li

Qi Zhang

Zhaowei Zhang

Jun Jiang

Fei Wang

Xiaoxia Ding

Du Wang

Xuefang Wang

Liangxiao Zhang

Edward Vernon Brush

Ming Li

Zheyu Zhang

Xiupin Wang

Huali Xie

Yizhen Bai

Zhe Yuan

Neil Martin Forcier

Hui Li

Tianxu Zhang

Cen Lu

Rui Wang

Xiaoqian Tang

Ao Zhong

Bernhard Holzinger

Xiaofeng Hu

Xin Li

Tao He

Zhiyong Zuo

Yisong Wang

Gang Zhou

Liangliang Wang

Beibei Yang

Ran Li

Lingkui Meng

Ziwen Gao

Xiaofeng Yue

Linyi Li

Zhaochu Hu

Changlu Cui

Changhui Yu

Haihong Chen

Yizhuo Meng

Chongxin Tao

Yongsheng Liu

A method for identifying and evaluating toxigenic capability of an aflatoxigenic strain. A ratio of the aflatoxin yield to Nor-1 gene transcriptional quantity is determined to have high relative stability. Anstrain toxigenic capability identification model is established, and thus a regression equation between thetoxigenic capability and the ratio AFT/Nor-1 of the aflatoxin yield to the Nor-1 gene transcriptional quantity is obtained.

Method for identifying and evaluating toxigenic capability of aflatoxigenic strain

A time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and an application thereof are disclosed. The kit comprises a time-resolved fluorescent immunochromatographic test strip and sample reaction vials each containing a europium-labeled monoclonal antibody lyophilized product; wherein the fluorescent test strip comprises a PVC substrate, and a surface of the PVC substrate is adhered with a water absorbing pad (), a detection pad () and a sample pad () from top to bottom, adjacent pads being connected and overlapping at connections. The detection pad () adopts a nitrocellulose membrane as the base thereof and is arranged with a lateral quality control line () and five detection lines () from top to bottom each covered by a bovine serum albumin conjugate of each toxin. The fumonisin B1 monoclonal antibody is generated by the hybridoma cell strain Fm7A11 having China Center for Type Culture Collection (CCTCC) accession number C201636. The kit is applicable to simultaneous detection of mixed contamination of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and sterigmatocystin.

Time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and application thereof

The present invention relates to an early warning method before the occurrence of aflatoxin contamination. The steps are as follows: extracting toxins from the sample to obtain a sample extract, and subjecting the sample extract to detection and analysis by liquid chromatography-high resolution mass spectrometer, performing qualitative analysis based on the mass spectrometry information to obtain qualitative results, performing quantitative analysis based on a standard curve of the chromatographic peak area of each warning molecule/the peak area of the internal standard-warning molecule concentration to obtain quantitative results of these warning molecules, wherein a risk of aflatoxin contamination of the sample is assessed to obtain a classification prediction model, inputting the quantitative results of the warning molecules for a toxigenic strain of, and outputting a risk assessment result based on the classification prediction model, thereby achieving the early warning before aflatoxin contamination occurs.

Early warning method before occurrence of aflatoxin contamination

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate. The three mycotoxins including 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin can be rapidly and synchronously detected.

Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1, and sterigmatocystin

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol, and T-2 toxin

The present disclosure discloses an intelligent point-of-care testing device for mycotoxins based on a quantum dots test strip. A first light source and a second light source are used for exciting a test strip which is placed in a shell and coated with a mycotoxin antigen to obtain emitted light, and a light filtering component is used for filtering out impurity light. A clamping mechanism is used for clamping and fixing an image capturing component. The image capturing component is used for capturing, an image of mycotoxins based on a quantum dots test strip. A processor is used for analyzing and calculating image information to obtain a mycotoxin content result. The testing time is shortened, and the portability and practicability of mycotoxin testing are improved. Quantitative instant testing of single or multi-toxin mixed pollution is achieved, and the technical problem of fluorescence quantification is solved.

Intelligent instant quantitative testing device for mycotoxin quantum dot test strip

A method of storing remote sensing big data in a Hbase database. The method includes: obtaining a remote sensing image, and pre-cropping the remote sensing image based on a Google S2 algorithm; selecting a grid level based on a resolution of the remote sensing image; demarcating a coverage scope of the remote sensing image; calculating grids covered within the coverage scope; cropping the remote sensing image according to the grids, to yield a plurality of image blocks, and serializing the plurality of image blocks; establishing an Hbase table; establishing a table storage model containing RowKey, and calculating the RowKey according to attributes of an image block; and integrating data structure of multi-source remote sensing image data according to attribute expression of a zoned remote sensing image in a plurality of dimensions, and storing serialized image blocks in an Hbase database according to the RowKey.

Method of storing remote sensing big data in Hbase database

The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.

Pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus

The present invention relates to the microbiological field, and particularly relates to aadcarboxglata biocontrol strain efficiently inhibiting production of aflatoxins by. Theadcarboxglata biocontrol strain Wt16 was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017331. Theadcarboxglata strain Wt16 is isolated from peanut pods for the first time which can significantly inhibit aflatoxins production by, and also has an extremely good effect on inhibiting aflatoxins production byfor peanuts from different sources.

biocontrol strain efficiently inhibiting production of aflatoxins byand application thereof

A system includes a controller that is configured to generate a plurality of switch control signals; a plurality of electrical circuit elements, the plurality of electrical circuit elements being characterized by a plurality of impedances, respectively; a plurality of voltage sources; and a plurality of switches that are programmable to couple the plurality of electrical circuit elements to the plurality of voltage sources responsive to the plurality of switch control signals.

Universal driver systems and methods of operating the same

The present invention provides an analysis method for determining halogens in geological samples by ICP-MS. The method includes following steps: weighing a geological sample and ammonium bifluoride in a sample dissolving tank, tightening the sample dissolving tank, and shaking; then heating the sample dissolving tank in a drying oven, and setting a temperature of the drying oven as 200-220&#xb0; C. and heating time as 1-2 hours; cooling the sample dissolving tank to room temperature so as to obtain a solid mixture after heating is ended, adding ammonium hydroxide into the solid mixture, centrifuging, removing a precipitate, and collecting the supernatant; adding an internal standard solution into the supernatant, and uniformly mixing; and optimizing the ICP-MS to an optimal state, testing content of chlorine in the supernatant under a condition of medium resolution m/&#x394;m=4000, and testing content of bromine and iodine in the supernatant under a condition of low resolution m/&#x394;m=300.

Analysis method for determining halogens in geological samples by ICP-MS

The invention discloses a NIR method for fatty acid content of oilseeds, including: selection of oilseed samples, and analyzing the oilseed samples by an near infrared spectrometer to obtain NIR spectra; preprocessing of NIR spectra and establishment of a NIR spectral database of oilseeds; establishment of a fatty acid database of oilseeds based on gas chromatography; establishment of prediction model of fatty acids in oilseeds; acquiring NIR spectrum of a sample to be tested by the near infrared spectrometer, and importing the preprocessed NIR spectrum into the prediction model of fatty acids to obtain the predicted fatty acid content of the tested sample. The method is simple and rapid to operate and is non-destructive, and the detection time of the sample is greatly shortened and the detection cost is reduced.

NIR spectroscopy method for fatty acid content of oilseeds

A method for multivariate adulteration detection on an edible oil includes (1) construction of a model: S1, acquiring near-infrared spectra of edible oils; S2, establishing a near-infrared spectral database of the edible oils; S3, establishing a multivariate adulteration detection model for a type of edible oil; and (2) application of the model: acquiring spectra of a sample to be tested according to the near-infrared spectral signal acquisition method in step S1, preprocessing the obtained near-infrared spectra by using the method in step S2 to obtain near-infrared spectral data of the sample, and determining the authenticity of the sample to be tested by using the multivariate adulteration detection model for the edible oil established in step S3. The method is simple and rapid in operation, can effectively and rapidly screen the authenticity of an edible vegetable oil, and has strong practicability.

Method for detecting multivariate adulteration of edible oil based on near-infrared spectroscopy

The illustrative embodiments pertain to a test fixture having low insertion inductance for large bandwidth monitoring of current signals. In one exemplary embodiment, the test fixture includes a baseplate with each resistor of a set of resistors embedded inside a respective non-plated through slot in the baseplate. A first terminal of each resistor is soldered to a top metallic zone of the baseplate and a second terminal soldered to a first of two bottom metallic zones of the baseplate. The top metallic zone is connected by plated-through holes to a second of the two bottom metallic zones. When mounted upon a PCB, the test fixture allows current flow from the first bottom metallic zone, upwards through the set of resistors to the top metallic zone, and downwards to the second bottom metallic zone. An observation instrument may be coupled to a coaxial connector that is mounted on the baseplate.

Test fixture for observing current flow through a set of resistors

An immunoadsorbent for purifying fumonisin B, aflatoxin B, ochratoxin A, zearalenone and sterigmatocystin; a complex affinity column and its preparation method; and a method for detecting the mycotoxins using the same are provided. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin Bmonoclonal antibody, an anti-aflatoxin Bmonoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin Bmonoclonal antibody is secreted by a hybridoma cell strain Fm7A11. The complex affinity column can be used for purification and detection of a fumonisin Bsample, an aflatoxin Bsample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

Immunoadsorbent for purifying five kinds of mycotoxins including fumonisin Band aflatoxin B, and complex affinity column

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Hubei, China

Wen Zhang