Beijing Transgen Biotech Co., Ltd.

Shanghai Huayi Acrylic Acid Co., Ltd.

Wen Xin

Liang Geng

Zhuang Yan

Zuo Linghua

Shao Jingming

Luo Ge

Chu Xiaodong

Zhao Xiaoqi

The present disclosure relates to a method for removing a non-target RNA from an RNA sample, including: performing reverse transcription on an RNA sample by using a reverse transcription primer, and removing an RNA template, to obtain a non-target first-strand cDNA and a target first-strand cDNA; hybridizing the non-target first-strand cDNA with a specific probe to obtain a non-target first-strand cDNA-probe complex; and digesting the non-target first-strand cDNA-probe complex by using a duplex-specific nuclease to obtain the target first-strand cDNA. The non-target first-strand cDNA-probe complex can be further extended to obtain a double-stranded DNA region that can be completely digested by the duplex-specific nuclease, then the duplex-specific nuclease is added for digestion. The present disclosure provides for simplicity, flexibility, and low costs, and can eliminate the impact of the non-target RNA on the downstream experiments, thereby having great and profound significance for research in the field of transcriptomics and RNA sequencing.

Method for removing non-target RNA from RNA sample

Growing community of inventors

Beijing, China

Wen Xin