Other

Tsinghua University

Wen-Ting Chen

Cheng-Yao Chen

Yi-Wen Cheng

Yu Ting Hung

Yu-Ting Hung

Hsien-Sheng Yin

Tsu-Ying Wu

Provided herein relates to DNA polymerase variants and kits including the same, where the DNA polymerase variant has an improved function and activity of performing template-independent nucleic acids synthesis using canonical nucleotides and non-canonical nucleotide analogues in a thermotolerant manner.

Polymerase variants for template-independent enzymatic nucleic acids synthesis and kit comprising the same

Disclosed herein is a specific B-family DNA polymerase variants that exhibit an improved incorporation of nucleotide analogues for synthesizing polynucleotides and sequencing the associated nucleic acid template. More particularly, the DNA sequencing-by-synthesis method can be efficiently performed by said B-family DNA polymerase variants with a normal nucleic acid template and reversible dye-terminator nucleotides to precisely determine the sequence of associated nucleic acid template.

B-family DNA polymerase variant and kit comprising the same

The present invention provides a mutant chicken interleukin-1&#x3b2; protein as a chicken interleukin-1&#x3b2; antagonist, which has a substituted peptide at position 117 and/or 118 of wild-type chicken interleukin-1&#x3b2; peptides. The mutant chicken interleukin-1&#x3b2; protein is created by using point mutation in a method genetic engineering; it can significantly inhibit proliferation of avian virus, such as avian influenza virus, avian reovirus and Marek's disease virus, and avian inflammation response. Therefore, the mutant chicken interleukin-1&#x3b2; protein of the present invention can be used as antiviral and anti-inflammatory medication.

Mutant chicken interleukin-1&#x3b2; protein as chicken interleukin-1&#x3b2; antagonist and uses thereof

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Hsinchu, Taiwan

Wen-Ting Chen