National University of Singapore

Illumina Corporation

Illumina Singapore Pte. Ltd.

Wen Shan Yew

Maybelle Darlene Kho Go

Sergio Peisajovich

Saurabh Nirantar

Jasmine Puay Suan Chua

A method for producing a cannabinoid by contacting cannabigerolic acid with a cannabinoid synthase orthologue. The cannabinoid synthase orthologue is from an organism other than. Also disclosed is a recombinant cell oforthat includes in its genome a nucleic acid encoding the above cannabinoid synthase orthologue. The cannabinoid synthase orthologue is expressed in the recombinant cell in an active form.

Biosynthesis of cannabinoids from cannabigerolic acid using novel cannabinoid synthases

Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His421 in the bovine TdT.

Thermostable terminal deoxynucleotidyl transferase

Growing community of inventors

Singapore, Singapore

Wen Shan Yew