Fujifilm Cellular Dynamics, Inc.

Cellular Dynamics International, Inc.

Wen Bo Wang

Casey Stankewicz

Steven James Kattman

Peter Fuhrken

Nathaniel Beardsley

Matt Riley

Maksym A Vodyanyk

Deepika Rajesh

Sarah Burton

Christie Munn

Bradley Swanson

Xin Zhang

Andrew J Brandl

Lucas Chase

Brad Swanson

Matt George

Carrie Chavez

Chris McMahon

Nathan Meyer

Matthew George

Christopher W McMahon

Lauren E Little

Nathaniel A Elliott

Ethan McLEOD

Nathaniel A Elliot

Steven Kattman

Provided herein are methods for the production of antigen-specific effector T cells and NK cells from pluripotent stem cells which express a chimeric antigen receptor (CAR). Further provided herein are methods for the adoptive cell therapy by administering the effector T cells and/or NK cells provided herein.

Antigen-specific immune effector cells

Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Methods for directed differentiation of pluripotent stem cells to immune cells

Methods are provided for efficient production of midbrain dopaminergic (DA) neurons. In some aspects, methods involve differentiation and selection of DA neurons for a transgenic pluripotent cell population (e.g., cells comprising a selectable marker gene). Cell populations produced by the instant methods and methods of their use are likewise provided.

Production of midbrain dopaminergic neurons and methods for the use thereof

Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.

Cardiomyocyte production

Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.

Methods for differentiating pluripotent cells

Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.

Titration of differentiation medium components

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Waunakee, WI, United States of America

Wen Bo Wang