Sun Yat-senuniversity

Other

Weiwei Su

Yonggang Wang

Wei Ping Peng

Qian Zhou

Chong Liu

Peibo Li

Ning Wang

Haibin Liu

Zhong Ming Wu

Fengyin Liang

Zhong Pei

Qin Deng

Xiao Shen

Mingyi Guan

Hongliang Yao

Minyi Guan

Zhong Wu

Yan Liao

Wei Peng

Haoyan Jiao

The present invention discloses a solution formulation for aerosol inhalation of naringenin and preparation method thereof. The formulation is prepared from 1 part by weight of naringenin, 15-30 parts by weight of hydroxypropyl β-cyclodextrin, a buffer-salt solution and an appropriate amount of an excipient. The preparation method includes: preparing a buffer-salt solution of a pH value of 7-8.5 by using the buffer salt, adding the naringenin into the buffer-salt solution, then adding the hydroxypropyl β-cyclodextrin, shaking in a constant-temperature air bath till complete dissolving and coating, adding an appropriate amount of the excipient, filtering, filling and sterilizing. The present invention, by firstly increasing the solubility of the free naringenin in the solvent by adjusting the pH value, and then coating the naringenin with the hydroxypropyl β-cyclodextrin, significantly increases the overall concentration of the naringenin in the solvent.

Method for preparing solution formulation for aerosol inhalation of naringenin

Disclosed is a new drug application of aBenth Extract (EA), and particularly is a method of the EA used for treating multiple diseases caused by drug resistant bacteria infection. Related drug resistant bacteria include a Multi-Drug Resistant (MDR)(MDRAB), an MDR(MDRPA), an Extended-Spectrum Beta-Lactamase (ESBL) producing(ECO) and an ESBL-producing(KPN).

Method of Chinese herbal medicine extract used for treating multiple diseases caused by drug resistant bacteria infection

Method of chinese herbal medicine extract used for treating multiple diseases caused by drug resistant bacteria infection

The disclosure relates to an expression method of a Haemocoagulase Acutus (Halase) recombinant protein. The method includes the following steps: (1) optimizing a halase gene; (2) performing Polymerase Chain Reaction (PCR) amplification on an optimized halase gene; (3) constructing an Agkis-pMCX expression vector, transforming plasmids to competent cells of anfor amplification, screening in an Amp-resistant manner for positive cloning, sequencing and extracting recombinant plasmids with correct sequencing; (4) transfecting recombinant plasmids to CHO cells; and (5) expressing the recombinant protein and identifying. According to the expression method, a recombinant halase is expressed first using the CHO cells cultured in a serum-free suspension manner; and by utilizing a bioengineering means, the actual production problems of insufficient raw material sources and unstable quality of a snake venom product are solved successfully.

Expression method of Haemocoagulase Acutus (Halase) recombinant protein

Provided herein is a method for preventing or treating neurodegenerative disease in a subject, comprising administrating to the subject an effective amount of a composition which comprises a semen biotae extract. The neurodegenerative disease is associated with &#x3b1;-synuclein, such as Alzheimer's disease, Parkinson's disease, Huntington disease, or Amyotrophic lateral sclerosis.

Method for preventing or treating neurodegenerative diseases

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Guangdong, China

Weiwei Su