Zhejiang Chiral Medicine Chemicals Co. Ltd.

Zhejiang University

Weirong Fan

Tianchun Liu

Youming Huang

Qin Zhang

Yuguo Zheng

Renchao Zheng

Jianfeng Weng

The disclosure provides a nitrilase from, which belongs to genus, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and &#x3e;99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.

Nitrilase from arabis alpina, its encoding gene, vector, recombinant bacterial strain and uses thereof

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This invention relates to an improved process for the preparation of substantially pure, stable anhydrous gabapentin. Thus, cyclohexane 1,1-diacetic acid monoamide is first treated with NaOH/NaClO to form 3,3-pentamethylenebutyrolactam, which is treated with aqueous HCl solution under reflux to provide gabapentin HCl salt, which is then neutralized with NaOH followed by a dehydration step to provide substantially pure, storage-stable, pharmaceutical grade gabapentin.

Process suitable for industrial scale production of gabapentin

Growing community of inventors

Hangzhou, China

Weirong Fan