Zhejiang University

Weiren Dong

Yan Yan

Gang Xing

Yanmei Zhang

Xiaoling Guo

Miaoan Shu

Jinyan Gu

Min Liao

Jiyong Zhou

Yulan Jin

Xiaojuan Zheng

The present disclosure provides an integrated rice-crayfish-oriental river prawn planting-breeding method. The method includes the following steps: stocking crayfish postlarvae in a paddy field as an integrated rice-crayfish-oriental river prawn farming area from February to March, catching and marketing the crayfish from May to June; planting in mid-June, sun-drying rice seedlings in the field in late June, cleaning and disinfecting a ring ditch surrounding the paddy field, and transplanting some aquatic plants; stocking oriental river prawn postlarvae in early July; harvesting ripe rice in late October; and catching and marketing the oriental river prawns in November. On the premise that the rice planting area is not reduced, the present disclosure realizes the effective expansion of time and space of the paddy field and improves comprehensive benefits of rice-fish farming in the form of integrated rice-crayfish-oriental river prawn planting-breeding.

Integrated rice-crayfish-oriental river prawn planting-breeding method

The present invention discloses a type II Pseudorabies virus attenuated strain and its preparation method and application. The attenuated strain of pseudorabies virus is gE/TK-double-deficient strain, which is named as PRV-HD/c strain of PRV dual-deletion strain, and the accession number is CGMCC No. 14325. The attenuated strain of the pseudorabies virus of the present invention is obtained from the newly isolated strain of pseudorabies virus type II after deletion of the gE and TK double genes and has reduced pathogenicity and strong immunogenicity and is inactivated by the attenuated strain of pseudorabies virus vaccines or live attenuated vaccines, which can provide effective immunity to PRV susceptible animals such as pigs and mice.

Type II pseudorabies virus attenuated strain, its preparation method and application

Growing community of inventors

Hangzhou, China

Weiren Dong