South Dakota State University

Johns Hopkins University

Weiping Zhang

David A Sack

David Francis

Provided herein are polypeptides comprising up to 9 antigenic elements of ETEC virulence determinants: 7 CFA adhesins [CFA/I, CFA/II (CS1, CS2, CS3), CFA/IV (CS4, CS5, CS6)] expressed by the most prevalent and virulent ETEC strains, and 2 toxins expressed by all ETEC strains, were genetically fused together for CFA-toxoid fusion with proteins (CFA/I/II/IV-STa-LT). Methods for making these polypeptides and their use in the treatment of ETEC related disease are also provided.

Multiepitope fusion antigens and vaccines and their use in treatment of enterotoxigenic diarrhea

Provided herein are polypeptides comprising up to 9 antigenic elements of ETEC virulence determinants: 7 CFA adhesins [CFA/I, CFA/II (CS1, CS2, CS3), CFA/IV (CS4, CS5, CS6)] expressed by the most prevalent and virulent ETEC strains, and 2 toxins expressed by all ETEC strains, were genetically fused together for CFA-toxoid fusion with proteins (CFA/I/II/IV-STa--LT). Methods for making these polypeptides and their use in the treatment of ETEC related disease are also provided.

Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic. The vaccine may include anstrain. Thestrain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain ofwith the genetic fusion.

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Brookings, SD, United States of America

Weiping Zhang